不同产地野生及栽培掌叶大黄中蒽醌类成分含量比较.docVIP

不同产地野生及栽培掌叶大黄中蒽醌类成分含量比较 　【摘要】 　　目的比较野生与栽培掌叶大黄中蒽醌类成分含量的差异。方法采用高效液相色谱法测定不同产地野生与栽培掌叶大黄中蒽醌类成分的含量，利用聚类分析对测定结果进行分类，通过相关分析研究海拔对掌叶大黄蒽醌类成分含量的影响。结果野生掌叶大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量范围分别为0.29%～0.67%，0.47%～1.33%，0.25%～0.87%，0.55%～1.00%，0.06%～0.48%；栽培掌叶大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量范围分别为0%～0.07%，0%～0.06%，0%～0.15%，0%～0.34%，0%～0.08%。聚类分析将野生掌叶大黄与栽培掌叶大黄明显地划为两类。相关分析结果表明，海拔高度对掌叶大黄蒽醌类成分含量有显著影响。结论野生掌叶大黄中蒽醌类成分的含量普遍高于栽培掌叶大黄。掌叶大黄中蒽醌类成分含量随海拔升高而升高。 【关键词】 掌叶大黄； 栽培品和野生品； 蒽醌； 高效液相色谱法 　　Abstract：ObjectiveTo do some comparative study on the contents of anthraquinones between the cultivated and wild samples of Rheum Palmatum. MethodsA reversed-phase HPLC method was established for the determination of contents of aloe-emodin，rhein，emodin，chrysophanol and physcion in cultivated and wild Rheum Palmatum from different regions. The classification was carried out by using the Hierarchical clustering analysis. The correlation analysis was used to study the effect of altitude on the contents of anthraquinones. ResultsThe content range of aloe-emodin，rhein，emodin，chrysophanol and physcion in wild Rheum Palmatum were 0.29％～0.67％，0.47％～1.33％，0.25％～0.87％，0.55％～1.00％，0.06％～0.48%, respectively. The content range of aloe-emodin，rhein，emodin，chrysophanol and physcion in cultivated Rheum Palmatum were 0～0.07％，0～0.06％，0～0.15％，0～0.34％，0～0.08％, respectively. According to the result of classification , all samples collected were divided into two grades the cultivated and the wild. The result of correlation analysis showed that the altitude had significant influence on the contents of anthraquinones in Rheum Palmatum. ConclusionThe contents of aloe-emodin，rhein，emodin，chrysophanol and physcion in the wild Rheum Palmatum are higher than those in the cultivated Rheum Palmatum. with the increase of altitude，the contents of anthraquinones show the tendency of increase. 　　Key words：Rheum palmatum L．; Cultivated and wild samples; Anthraquinones; HPLC 　　掌叶大黄Rheum palmatum L．为蓼科大黄属多年生草本植物，以干燥根及根茎入药，是药典中大黄药材的主要来源之一