乳腺癌中STAT3对HSP27及c.docVIP

乳腺癌中STAT3对HSP27及c 　【关键词】 乳腺癌 　　Regulating role of STAT3 to HSP27 and cMYC expression in breast cancer 　　【Abstract】 AIM: To investigate the regulating role of signal transducer and activator of transcription 3 (STAT3) to 27 ku heat shock protein (HSP27) and cMYC expression in breast cancer. METHODS: Expressions of STAT3, HSP27 and cMYC were examined in primary breast infiltrating duct carcinoma tissues of 51 patients using immunohistochemistry. Correlations between STAT3 expression and HSP27 or cMYC expression were analyzed with statistic method. After the transfection of MDAMB435S human breast carcinoma cells with STAT3 decoy oligodeoxynucleotides (Decoy ODNs), expressions of HSP27 and cMYC of MDAMB435S were semiquantified by Western blot, the apoptosis and the cell cycle distribution of MDAMB435S cells were analyzed with flow cytometry and proliferation of MDAMB435S cells was analyzed by MTT assay. RESULTS: In primary breast infiltrating duct carcinoma tissues, STAT3 expression was correlated positively with HSP27 expression and cMYC expression respectively (rs=0.454, P=0.001; rs=0.541, P=0.000). As the result of STAT3 inhibition by STAT3 Decoy ODNs in MDAMB435S cells, expressions of HSP27 and cMYC were markedly reduced, which was consistent with STAT3 activation reduction (Both Plt;0.01). The cell cycle progression of MDAMB435S cells was significantly inhibited at G0/G1 phase, the apoptosis of those cells was augmented and their proliferation was inhibited (Plt;0.01). CONCLUSION: Constitutively activated STAT3 may upregulate HSP27 and cMYC expression to inhibit breast cancer cell apoptosis and to facilitate their proliferation, thus leading to the malignancy of breast cancer cells. 　　【Keywords】 breast neoplasms; oncogenes; signal transducer and activator of transcription 3; heat shock proteins 27; genes, cMYC 　　【摘要】 目的：研究人乳腺癌组织中信号转导和转录激活因子3(STAT3)对热休克蛋白27（HSP27）和cMYC的调控作用. 方法：应用免疫组化检测51例人乳腺癌组织STAT3，HSP27，cMYC蛋白的表达，分析他们的相互关系及与临床预后相关指标的关系. 在体外应用诱骗寡核苷酸（Decoy ODNs）抑制乳