二维三七桂利嗪胶囊对急性脑缺血保护作用.docVIP

二维三七桂利嗪胶囊对急性脑缺血保护作用 　 作者：肖湘华，邓秀玲，王燕，刘俊田，王竹筠; 【关键词】; 急性脑缺血 毕业论文 　　摘要：目的; 观察二维三七桂利嗪胶囊对于急性脑缺血的影响。方法; ICR小鼠分别用420mg/kg、210mg/kg、105mg/kg的二维三七桂利嗪胶囊灌胃10d，观察断头后小鼠的喘息时间；SD大鼠分别用292mg/kg、146mg/kg、73mg/kg的二维三七桂利嗪胶囊灌胃10d，线栓法制备大鼠大脑中动脉栓塞模型，然后进行神经行为指标评分、脑梗死范围测定和病理学观察。结果; 二维三七桂利嗪胶囊各剂量组均能延长小鼠断头后的喘息时间，缩小局灶性脑缺血大鼠脑梗死范围，改善神经症状，减轻光镜下脑组织缺血性损伤。结论; 二维三七桂利嗪胶囊对急性脑缺血损伤具有保护作用。 毕业论文 　　关键词：二维三七桂利嗪胶囊；急性脑缺血；神经保护作用 　　 　　ABSTRACT: Objective; To investigate the effects of divitamins notonginseng and cinnarizine capsule(DNCC) on acute cerebral ischemia. Methods; ICR mice were administered three doses of DNCC(420, 210, and 105mg/kg; ig.) for ten days, then the gasping time after decapitation was recorded. After three doses of DNCC(292, 146, and 73mg/kg; ig.) were given respectively for ten days in rats, the effects of DNCC on the infarct size, histological changes and neurological function scores caused by focal cerebral ischemia which was induced by middle cerebral artery occlusion were investigated. Results; DNCC prolonged the gasping time of mice after decapitation and improved the neurological function scores, cerebral ischemia injury and decreased the infarct size in rats. Conclusion; DNCC has a protective effect against acute cerebral ischemia. 毕业论文 　　KEY WORDS: divitamins notonginseng and cinnarizine capsule; acute cerebral ischemia; neuroprotective effect 毕业论文 　　 二维三七桂利嗪胶囊(divitamins notonginseng and cinnarizine capsule, DNCC)是由桂利嗪、三七总皂苷、维生素E和维生素B6组成的中西药复方制剂，临床主要用于治疗缺血性脑血管病。实验证明钙通道阻滞剂能对抗缺血后脑内的低氧高糖状态，缩小脑梗死范围。三七总皂苷能减少大脑中动脉栓塞(middle cerebral artery occlusion, MCAO)术后24h脑梗死范围，改善神经功能障碍及行为异常，减轻脑水肿，明显提高SOD活力，减轻脑梗死区的炎症反应。维生素E能提高缺血后机体的抗氧化能力，缩小脑梗死范围。但以上述成份组成的药物――DNCC对于脑缺血的影响尚未见报道。我们选择DNCC作为受试药物，分别采用小鼠断头法和线栓法诱导大鼠MCAO造成急性脑缺血模型，观察DNCC对于急性脑缺血的影响。 毕业论文 　　1; 材料与方法 毕业论文 　　1.1; 动物、药品与试剂; ICR小鼠，雌雄各半，体重18-22g；SD大鼠，雄性，体重200-300g，均由西安交通大学医学院实验动物中心提供。自然光周期饲养，大鼠术前8h禁食，自由饮水。二维三七桂利嗪胶囊(DNCC)，批号为030608；单组份桂利嗪(cinnarizine)，批号030651；单组份参三七提取物(extracts of panax notoginseng, EPN)，内含三七总皂苷54.46%，批号030506，以上药品均由丽珠集团