原创力文档14-3-3epsilon contributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion在喉部癌14-3-3epsilon有助于肿瘤抑制影响细胞凋亡和入侵.pdfVIP
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14-3-3epsilon contributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion在喉部癌14-3-3epsilon有助于肿瘤抑制影响细胞凋亡和入侵
Che et al. BMC Cancer 2010, 10:306
/1471-2407/10/306
R E S E A R C H A R T I C L E Open Access
Research article
14-3-3epsiloncontributes to tumour suppression in
laryngeal carcinoma by affecting apoptosis and
invasion
1 1 2 3 1 1
Xing-Hua Che , Hong Chen , Zhen-Ming Xu , Chao Shang , Kai-Lai Sun and Wei-Neng Fu*
Abstract
Background: 14-3-3epsilon regulates a wide range of biological processes, including cell cycle control, proliferation,
and apoptosis, and plays a significant role in neurogenesis and the formation of malignant tumours. However, the
exact function and regulatory mechanism of 14-3-3epsilon in carcinogenesis have not been elucidated.
Methods: The expression of 14-3-3epsilonwas assessed by RT-PCR and western blotting. The invasiveness and viability
of Hep-2 cells were determined by the transwell migration assay and MTT assay, respectively. Cell cycle and apoptosis
of Hep-2 cells were detected by flow cytometry.
Results: The mRNA and protein expression of 14-3-3epsilon in larynx squamous cell carcinoma (LSCC) tissues were
significantly lower than those in clear surgical margin tissues. Statistical analysis showed that the 14-3-3epsilon protein
level in metastatic lymph nodes was lower than that in paired tumour tissues. In addition, the protein level of 14-3-
3epsilon in stage III or IV tumours was significantly lower than that in stage I or II tumours. Compared with control Hep-
2 cells, the percentages of viable cells in the 14-3-3epsilon-GFP and negative control GFP groups were 36.68 ± 14.09%
and 71.68 ± 12.10%, respectively. The proportions of S phase were 22.47 ± 3.36%, 28.17 ± 3.97% and 46.15 ± 6.82%, and
the apoptotic sub-G1 populations were 1.23 ± 1.02%, 2.92 ± 1.59% and 13.72 ± 3.89% in the co
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