哮喘儿童外周血T淋巴细胞Fas-FasL表达及其意义.docVIP

哮喘儿童外周血T淋巴细胞Fas/FasL表达及其意义 　 作者：袁岳沙 陈杰 王琳 周少华 【摘要】 　　目的探讨哮喘儿童外周血T淋巴细胞Fas、FasL表达及其意义。方法21例哮喘发作期患儿、15例哮喘缓解期患儿及25例正常儿童作为研究对象；密度梯度法分离外周血淋巴细胞；免疫荧光抗体法检测淋巴细胞Fas、FasL表达阳性率；用t检验和直线相关分析进行资料统计处理。结果发作期患儿淋巴细胞Fas表达明显下降，与正常对照组和缓解期患儿组比较差异均有非常显著意义（t=5.914 2， 3.108 4, Plt;0.01）；缓解期患儿淋巴细胞Fas表达比发作期患儿有明显提高，但与正常组相比仍有显著降低（t=2.338 8, Plt;0.05）；淋巴细胞FasL表达总体均较低，但在发作期患儿仍明显高于正常对照组（t=2.186 1, Plt;0.05），在缓解期患儿FasL表达虽高于正常对照组，但无统计学意义（t=1.772 4, Pgt;0.05）；发作期患儿外周血中表达Fas的淋巴细胞阳性率与淋巴细胞比率呈明显的负相关（r=-0.632, Plt;0.05），而表达FasL的淋巴细胞阳性率与淋巴细胞比率间无明显相关性。结论哮喘发作期和缓解期儿童存在淋巴细胞凋亡下降，这可能是哮喘患儿气道慢性炎症持续存在的原因；同时提示在缓解期患儿仍需继续维持治疗。 【关键词】 哮喘 儿童 淋巴细胞 Fas(CD95) FasL 　　Abstract：ObjectiveTo investigated the expression of Fas/FasL on peripheral blood T cells in asthmatic children and to explore the relationship between its clinical significance. MethodsLymphocytes were isolated with ficoll-hypaque gradient method and T lymphocytes were further purified with nylon pili. Expression of Fas/FasL was detected by immunofluoresence technology.ResultsExpression of Fas on T cells in 21 cases of the stage of attack (5.81±3.29) and in 15 cases of remission stage (9.86±4.54) were remarkably lower than those in 25 healthy children (13.72±5.33) (Plt;0.01). Expression of FasL on T cells in the stage of attack (3.22±1.74) was significantly increased compared with normal control group (2.31±1.05) (Plt;0.05) but those in remission patients (2.97±1.28) was not significantly changed. There was a remarkable negative relationship between expression of Fas and percentages of peripheral blood lymphocyte (r=-0.632, Plt;0.01).ConclusionThe apoptosis of peripheral blood T lymphocytes from the children with asthma both in stage of attack and remission are decreased. The inhibited apoptosis might be one of reasons for the chronic persistent inflammation in the airways in asthmatic children and suggests that it is necessary to continue therapy in remission stage. 　　Key words： Asthma； Child； Lymphocyte ； Fas； FasL 　　支气管哮喘以可逆性气道阻塞、气道高反应性、气道炎症为其三大主要特征，其中以气道炎症为