复方中药膜分离工艺及节能性探究.docVIP

复方中药膜分离工艺及节能性探究 　【摘要】 　　目的利用不同规格超滤膜对复方中药丹参芍药水煎液进行分离和反渗透浓缩，考察其有效部位分离的最适条件参数和浓缩节能性。方法采用正交设计，以复方有效成分丹参酚酸和芍药苷转移率为指标，考察提取液pH值、温度、操作压力对膜分离的影响，并比较反渗透膜浓缩与减压浓缩对其质量和能量消耗的影响。结果以10 000Da的超滤膜分离时获得的产品纯度高，损失小。最佳分离条件为：pH 5，温度40℃，操作压力0.3 MPa。反渗透膜浓缩产品质量显著改善，有效成分的保留量高于减压浓缩的1倍以上，而其直接能耗只有其1/10，时间为其1/5。结论膜分离和浓缩用于复方中药的分离是可行的，可显著改善品质和节约能源。 【关键词】 膜分离 反渗透浓缩 复方中药 节能 　　Abstract：ObjectiveTo find out the optimum conditions of separation by ultra filter and energy efficiency by reverse osmosis in complex Chinese medicines. MethodsEffective parts from the complex of Salvia miltiorrhiza and Paeonia Lactiflora were extracted with water, then passed through ultra filter. Optimum ultra filter separating conditions such as pH, temperature and operating pressure were observed by evaluation based on total transfer rate of salvianolic acids and paeoniflorin. Energy efficiency in concentration was discussed in terms of direct energy consumption. Results10000Da ultra filter membrane increased the purity of product and decreased the loss, better quality of the extract was obtained if it was ultrafiltered in pH5, 40℃ with pressure of 0.3MPa.Compared with vacuum vaporization, reverse osmosis improved the quality of extract significantly with more than one times higher level of effective parts, 1/10 energy and 1/5 time consumption. ConclusionIt is practicable that membrane separation and concentration are used in the extraction of effective parts from complex Chinese medicines. 　　Key words：Membrane separation; Reverse osmosis; Complex Chinese medicine; Energy efficiency 　　膜分离技术能够有效地去除悬浮物、蛋白、色素、果胶等大分子物质，取代醇沉［1，2］，因而被广泛应用于制药工业中。但是目前利用膜分离技术，只是对单一组分进行考察，而对多种组分的复方则少有报道。由于复方有效部位是由多成分组成的群体，只有将各成分的含量变化进行综合考察，才能客观反映分离工艺的优劣［3］。 　　中药提取液的浓缩是中药制药的重要工序之一，对药品质量和能耗均有较大的影响［4］。反渗透膜浓缩与传统浓缩分离方法相比，有常温下操作、分离效率高、对有效成分破坏较少的优点［5］。本实验以丹参芍药组成的复方水煎液为研究对象，由于丹参酚酸和芍药苷均为有效成分，故以两者总的转移率为指标来进行膜分离工艺参数考察，探讨该技术用于中药复方有效部位分离提取的可行性以及节能优势。 　　1 器材 　　1.1 药材和试剂 　　丹参药材为植物丹参Salvia miltiorrhiza Bge.的根，湖北产；芍药药材为植物芍药Paeonia Lactiflora Pall的根，浙江产，购自广州南北行中药饮片有限