复方清脂颗粒对大鼠肝毒性实验探究.docVIP

复方清脂颗粒对大鼠肝毒性实验探究 　【摘要】 　　目的 研究复方清脂颗粒对大鼠肝脏的毒性作用。方法大鼠连续灌胃给予复方清脂颗粒生药52，26和13 g/kg连续8周，同时设对照组。禁食12 h后用水合氯醛麻醉动物，取肝脏称重并计算肝系数，光镜下做病理组织学检查，并测定血清中AST，ALT，ALP和肝组织中SOD和MDA。结果复方清脂颗粒52 g/kg组大鼠的肝重、肝系数、肝组织中MDA量都明显高于对照组，但SOD活力与对照组之间没有明显差别，26 g/kg组肝系数和肝组织中MDA高于对照组，13 g/kg组所有指标与对照组之间均无显著差异。病理组织学检查各组均未见肝组织有明显病变。结论长期给予复方清脂颗粒52，26 g/kg，对肝脏有一定毒性但没有造成肝脏的器质性损伤，13 g/kg是无毒性作用剂量。 【关键词】 大鼠；肝毒性；超氧化物歧化酶；丙二醛 　　Abstract:ObjectiveTo study the side-effects of Fufang Qingzhi(FFQZ)granules in rat liver. MethodsRats were intragastrically treated with Fufang Qingzhi granules(52, 26, 13g/kg) once a day for 8 weeks and the control group with water.Rats were anesthesia by chloral hydrate after fasting for 12 hours. Liver weight was measured, and liver coefficient was calculated. Histopathological examination for the liver was also performed with light microscopt.Activities of aspartate amino transferase (AST),alanine aminotransferase (ALT),alkaline phosphatase (ALP) in serum and activity of superoxide dismutase(SOD)and concentration of malondialdehyde(MDA) in liver tissue were determined.ResultsLiver weight ,liver coefficient and concentration of MDA increased significantly in rats between FFQZ group of 52g/kg and control group,but activity of SOD was no different from controls. Liver coefficient and concentration of MDA increased significantly in rats with FFQZ group of 26g/kg .No significant changes in indicators were found in the rats with FFQZ group of 13g/kg compared with control group. There were no histopathological changes of the liver in all rats.ConclusionBy long-term administration,FFQZ of 52 and 26g/kg has some hepatotoxicity in rats,but no histopathological changes were observed in liver. 13g/kg is the nontoxic dose. 　　Key words:Rats; Hepatotoxicity; Superoxide dismutase ; Malondialdehyde 复方清脂颗粒是由决明子、泽泻、姜黄、首乌等组成的中药复方，经药效学研究证明能显著降低血脂，大鼠药效学有效剂量为5 g/kg。我们进行了复方清脂颗粒大鼠肝毒性的实验研究。现报道如下。 　　1 器材与方法 　　1.1 药品和仪器 　　复方清脂颗粒，天津中医药大学中医药研究院药学部提供，批号天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）试剂盒，中生北控生物科技股