夏至草醇提物对实验性弥散性血管内凝血大鼠心肌损伤影响.docVIP

夏至草醇提物对实验性弥散性血管内凝血大鼠心肌损伤影响 　 作者：梁海峰，王伟平，张玉平，杜舒婷，姜华 【摘要】 　　目的观察夏至草醇提物对高分子右旋糖酐（Dextran 500）致大鼠实验性弥散性血管内凝血（DIC）心肌损伤的影响。方法Wistar雄性大鼠20只，随机分为夏至草组（n=8）、模型组（n=6）和对照组（n=6）。静脉推注10% Dextran 500（10 ml/kg.bw）复制DIC模型（对照组以等量生理盐水代替）。6 min后，夏至草组自颈静脉缓慢推注夏至草醇提物（5 g/ml，6g/kg.bw），其它两组以等量生理盐水代替。40 min后，制备心肌组织匀浆，观察心肌匀浆MDA含量及SOD活性、NO含量及NOS活性的变化。结果与对照组相比，模型组心肌匀浆MDA，NO含量及NOS活性显著升高，SOD活性降低；夏至草组心肌匀浆MDA，NO含量及NOS活性显著低于模型组，SOD活性升高。结论夏至草醇提物减轻Dextran 500致DIC大鼠心肌损伤的机制与降低自由基损伤、降低NO的生成与释放有关。 【关键词】 夏至草； 弥散性血管内凝血； 心肌损伤； 自由基； 一氧化氮 Abstract：ObjectiveTo observe the effects of ethanol extract from Mrrubium incisum on myocardium injury of disseminated intravascuclar coagulation (DIC) rats induced by Dextran 500. Methods20 male Wistar rats were divided into three groups: Mrrubium incisum group (n=8), model group (n=6) and control group (n=6). The DIC model was established by intravenous injection of Dextran 500 (10ml/kg.bw), and Dextran 500 was replaced by equivalent normal saline in control group. 6 minutes later, ethanol extract from Mrrubium incisum was infused (5g/ml, 6g/kg.bw); in other groups ethanol extract from Mrrubium incisum was replaced by normal saline. After 40 min, the hearts were taken out, 10% homogenate was prepared, and the free radical indexes of MDA content and SOD activity, NO content and NOS activity were determined. Results The contents of MDA and NO and NOS activity of myocardium homogenate in model group were significantly increased, and SOD activity was significantly lowered than that of control group. The contents of MDA and NO and NOS activity of myocardium homogenate in Mrrubium incisum group were significantly lowered, and SOD activity were significantly increased as compared with model group.ConclusionEthanol extract from Mrrubium incisum could markedly abate myocardium injury of DIC rats induced by Dextran 500 and its mechanism may be related to its antiperocidation and decreasing release of NO. 　　Key words：Mrrubium incisum; Disseminated intravascuclar coagulation (DIC); Myo