夏至草醇提物干预实验性弥散性血管内凝血大鼠肺损伤实验探究.docVIP

夏至草醇提物干预实验性弥散性血管内凝血大鼠肺损伤实验探究 　 作者：晁怀宇，张玉平，刘正泉，雷慧，杜舒婷，姜华 【摘要】 　　目的观察夏至草醇提物对高分子右旋糖酐（Dextran 500）致弥散性血管内凝血（DIC）大鼠肺损伤的干预作用。方法Wistar雄性大鼠20只，随机分为夏至草组（n=8）、模型组（n=6）和对照组（n=6）。静脉推注10%Dextran 500（10 ml/kg·体重）复制DIC模型（对照组以等量生理盐水代替）。6 min后，夏至草组自颈静脉缓慢推注夏至草醇提物（5 g/ml，6 g/kg·体重），其它两组以等量生理盐水代替。40 min后，制备肺组织匀浆，观察肺匀浆自由基指标MDA含量及SOD活性、NO含量及NOS活性的变化。结果与对照组相比，模型组肺组织匀浆MDA、NO含量及NOS活性显著升高，SOD活性降低；夏至草组肺组织匀浆MDA、NO含量及NOS活性显著低于模型组，SOD活性升高，各指标与对照组比较无统计学意义。结论夏至草醇提物减轻Dextran 500致DIC大鼠肺损伤的机制与降低自由基损伤、降低NO的生成与释放有关。 【关键词】 夏至草；弥散性血管内凝血；肺损伤；自由基；一氧 　　Abstract：ObjectiveTo observe the effects of ethanol extract from Mrrubium incisum against lung injury of disseminated intravascuclar coagulation (DIC) rats induced by Dextran 500.Methods20 male Wistar rats were randomly divided into three groups: Mrrubium incisum group (n=8), model group (n=6) and control group (n=6). The DIC model was established by intravenous injection of Dextran 500 (10 ml/kg·bw), and Dextran 500 was replaced by equivalent normal saline in control group. 6 minutes later, ethanol extract from Mrrubium incisum was infused (5 g/ml, 6g/kg·bw); in other groups ethanol extract from Mrrubium incisum was replaced by normal saline. After 40 min, the lung was taken out, the 10% tissue homogenate was prepared, and the free radical indexes of MDA content and SOD activity, NO content and NOS activity were determined.ResultsThe contents of MDA and NO and NOS activity of lung homogenate in model group were significantly increased, and SOD activity were significantly lower than that in control group. The contents of MDA and NO and NOS activity of lung homogenate in Mrrubium incisum group were significantly lowered, and SOD activity were significantly increased as compared with model group. There was no statistical difference between Mrrubium incisum group and control group. ConclusionEthanol extract from Mrrubium incisum can markedly abate lung injury of DIC rats by Dextran 500 and its mechanism may be related to scavenging free radical and decreasing N