小凹蛋白在清道夫受体AI介导巨噬细胞源性泡沫细胞形成过程中.docVIP

小凹蛋白在清道夫受体AI介导巨噬细胞源性泡沫细胞形成过程中 　 作者：王蓉蓉，朱炳阳，严鹏科，廖端芳 【关键词】 小凹;蛋白；清道夫受体AI；氧化性低密度脂蛋白；巨噬细胞；泡沫细胞 　　Role of caveolin1 in SRAI mediated formation of foam cells derived from macrophages 　　【Abstract】 AIM： To investigate the role of caveolin1 in scavenger receptor ai (SRAI) mediated formation of macrophagederived foam cells induced by oxidized lowdensity lipoprotein (oxLDL). METHODS: RAW 264.7 macrophages were treated with 75 mg/L oxLDL for different hours. Next, macrophages were pretreated with SB202190 and SRAI antisense oligonucleotides before 75 mg/L oxLDL treatment for 24 h. Western blot analysis was used to detect protein expression. High performance liquid chromatography (HLPC) analysis was performed to determine the cellular cholesterol content. Oil red O staining was used to show the lipid droplets in foam cells. RESULTS: Western blot analysis showed that SRAI expression increased with 75 mg/L oxLDL treatment for 12, 24 and 48 h. 75 mg/L oxLDL treatment for 6, 12, 24, 48 and 96 h decreased the expression of caveolin1. When oxLDL treatment was over 24 h, descended expression of caveolin1 in macrophages was observed and lasted for 96 h. Pretreatment of SB202190 and SRAI antisense oligonucleotides decreased the expression of SRAI and increased the expression of caveolin1. Meanwhile, the cellular cholesterol content was also decreased (Plt;0.01 vs oxLDL treatment groups). Oil red O staining showed that the SB202190 pretreatment group and SRAI antisense oligonucleotides group formed less lipid droplets than oxLDL treatment group did. CONCLUSION: There appears a negative relationship between caveolin1 and SRAI during the accumulation of lipidladen foam cells. 　　【Keywords】 Caveolae; caveolin1; Scavenger receptor AI; Atherosclerosis; Oxidized lowdensity lipoprotein; cholesterol; macrophage 　　【摘要】 目的：研究小凹蛋白在清道夫受体AI介导的巨噬细胞源性泡沫细胞形成过程中的作用. 方法：用75 mg/L氧化型低密度脂蛋白(oxLDL)与巨噬细胞共同孵育不同时间. 清道夫受体AI反义寡核苷酸、P38阻断剂SB202190预处理巨噬细胞，然后给予75 mg/L oxLDL处理24 h. Western blot法检测清道夫受体AI和小凹蛋白的表达. 高效液相色谱法