扶正软坚方对肺纤维化大鼠基质金属蛋白酶及其抑制因子表达影响.doc

扶正软坚方对肺纤维化大鼠基质金属蛋白酶及其抑制因子表达影响 　 作者：季旭明，赵启韬，孙敬昌，杨勇，朱庆均，刘恒，郑广娟 【摘要】 目的 研究 扶正软坚方对肺纤维化的干预作用及对MMP-9和TIMP-1表达的 影响 。 方法 Wistar雄性大鼠60只，随机分为空白组、模型组、扶正软坚方大剂量组（简称“大剂量组”）、扶正软坚方小剂量组（简称“小剂量组”）。博莱霉素诱发大鼠肺纤维化建立模型，第28天时取肺组织行HE染色，观察病理形态学改变；运用免疫荧光方法，激光共聚焦显微镜检测肺组织MMP-9和TIMP-1的表达，并进行半定量 分析 。结果 与空白组比较，模型组MMP-9表达明显降低（Plt;0.05）,TIMP-1表达明显增强（Plt;0.01）；扶正软坚方各组能明显增强MMP-9的表达（Plt;0.05），明显降低TIMP-1的表达（Plt;0.01）。结论 扶正软坚方能减轻博来霉素诱导的大鼠肺纤维化，作用机制可能是通过增强MMP-9蛋白表达，抑制TIMP-1蛋白表达实现。 【关键词】 扶正软坚方；肺纤维化；基质金属蛋白酶-9；基质金属蛋白酶抑制剂-1 论文代写 【Abstract】 Objective To study the effect of Fuzhengruanjian prescriptions on pulmonary fibrosis in rats and its possible mechanism.Methods Sixty male Wistar rats were randomly divided into four groups: normal group, model group, large-dose group and little-dose group. The rat model was induced by bleomycin. HE staining was detected and pathomorphology was observed. The level of MMP-9 and TIMP-1 were detected by immuno fluorescence technique and laser scanning confocal microscopy on 28th day after the treatment.Results In model group rats, the expression of MMP-9 was significantly degraded and the expression of TIMP-1 was significantly upgraded compared with that of normal group（Plt;0.05）. Fuzhengruanjian prescriptions could significantly upgrade the expressions of MMP-9 and degrade the expressions of TIMP-1 in a dose-dependent way.Conclusion Fuzhengruanjian prescriptions alleviated bleomycin-induced pulmonary fibrosis in rats, increasing the expression of MMP-9 and suppressing the expression of TIMP-1 should be the mechanisms. 毕业论文 【Key words】 Fuzhengruanjian prescription；pulmonary fibrosis；matrix metalloprotEinase-9；inhibitior of matrix metalloprotEInase-1 论文代写 肺纤维化是多种病因引起的一种慢性炎症性间质性肺疾病，其主要病理改变为弥漫性肺泡炎、肺泡单位结构紊乱和肺纤维化。基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶抑制剂-1（TIMP-1）与肺纤维化 发展 关系密切，TIMP-1在肺纤维化过程中高表达，成为肺纤维化的标志物之一。笔者以大鼠肺纤维化模型为研究对象，探讨扶正软坚方对肺纤维化的 治疗 作用及对MMP-9和TIMP-1表达的影响，结果报道如下。 1 材料与方法 论文代写 1.1 实验动物及分组 Wistar健康雄性大鼠60只，体重180～200g，购于山东中医药大学实验动物中心。实验大鼠随机分为空白组、模型组、扶正软坚方大剂量