抗血管内皮生长因子抗体对肝癌放射增敏作用.doc

抗血管内皮生长因子抗体对肝癌放射增敏作用 　 作者：郑青平,陈龙华,石玉生 【关键词】 肝肿瘤;放射疗法;抗VEGF单抗;放射增敏 　　Radiosensibility of human hepatocellular carcinoma xenografts enhanced by antivascular endothelial growth factor monoclonal antibody 　　【Abstract】 AIM: To investigate the inhibiting effects of combining antivascular endothelial growth factor (VEGF) monoclonal antibody (mAb) therapy with radiotherapy on the growth of hepatocellular carcinoma xenografts in vivo. METHODS: Human hepatocellular carcinoma cell line, SMMC7721, was implanted sc in mice. The mice with xenografts were divided into 4 groups, radiation (RT) 20 Gy group, RT 20 Gy plus mAb group, RT 30 Gy plus mAb group and control group. AntiVEGF mAb was injected ip on alternate days for a total of 6 injections at a dosage of 50 μg/mouse. Single radiation doses were given 24 h after the 4th injection of mAb. Tumor diameters were measured and tumor volume was calculated. Mice were sacrificed 2 wk after the 6th injection and the intratumoral microvessel density was determined by counting the stained vessels with immunohistochemistry. RESULTS: Radiation significantly reduced the growth and the microvessel density of tumors. The inhibitory effects were more significant when radiation was combined with antiVEGF mAb, and RT 30 Gy plus mAb was more effective than RT 20 Gy plus mAb. For RT 20 Gy group, RT 20 Gy plus mAb group and RT 30 Gy plus mAb group, the inhibitory rates of tumor weight were 75.3%, 83.9% and 94.7%, respectively. CONCLUSION: AntiVEGF mAb enhances the radiosensibility of human hepatocellular carcinoma xenografts.The strategy is one of the approaches for hepatocellular carcinoma treatment. 　　【Keywords】 hepatocellular carcinoma; radiotherapy; antiVEGF mAb; radiosensibility 　　【摘要】 目的: 观察抗血管内皮生长因子抗体(VEGF mAb)与不同剂量放射联合对肝癌裸鼠移植瘤生长的抑制作用. 方法: 将SMMC7721肝癌细胞种植于裸鼠皮下,成瘤动物分为单纯放射20 Gy组、放射20 Gy+抗体组、放射30 Gy+抗体组和对照组,腹腔给予抗VEGF mAb 50 μg/只，隔日1次,共6次,放射剂量一次性给予.测量肿瘤直径并计算瘤体积,抗体给药结束后2 wk处死动物,免疫组化法测肿瘤微血管密度(MVD). 结果: 放射能抑制肿瘤生长,减少肿瘤MVD,放射与抗体结合对肿瘤生长的抑制作用更显著，且放射30