海鞘对实验性糖尿病大鼠非酶糖基化影响.docVIP

海鞘对实验性糖尿病大鼠非酶糖基化影响 　【摘要】 目的 证实海鞘治疗糖尿病的有效性以及探讨其作用机制。方法 应用链尿菌素腹腔注射制作大鼠糖尿病模型，1 w后检查其空腹血糖，血糖水平超过8.1 mmol/L的大鼠被随机分为3 组：海鞘组，优降糖（阳性对照药）组，糖尿病对照组。另取10 只大鼠作为空白对照组。连续给药4 w后应用采取尾静脉血检测其血糖水平；血浆果糖胺和Hb糖化值及胰腺、肾和肝脏蛋白糖化值。结果 注射链脲菌素大鼠的血糖水平均显著高于正常对照组。连续给药4 w后海鞘和优降糖组，模型对照组血糖明显高于空白对照组，海鞘和优降糖组明显低于模型对照组。血浆、红细胞、肝、胰腺和肾脏蛋白糖化值明显低于模型对照组。结论 海鞘具有明显的降糖作用，对高血糖引起的血浆蛋白和肝脏、肾脏、胰腺组织蛋白的非酶糖基化有明显的抑制作用。 【关键词】 海鞘 血糖 蛋白糖基化 动物实验 　　Abstract: Objective To confirm the effectiveness of ascidians on diabetes and to probe into its functional mechanism. Methods The mice were injected intraperitonealy with streptozotocin (STZ) to be used as experimental models of diabetic mice. Having been tested the fasting blood glucose after one week, the mice whose blood glucose were over 8.1mmol/L were divided into three groups randomly: ascidians group, Youjiangtang(positive control drug) group, diabete control group. Another ten mice were chosen as blank control group. After 4 weeks, being continuously administrated, the mice were tested the blood glucose by adopting tail vain blood; plasma fructosamine, Hb diastatic value, and kidney and liver protein diastatic value. Results The blood glucose level of mice injected streptozotocen (STZ) was obviously higher than that of normal control group. The level of blood glucose of each group after 4 weeks, continuous administration indicated: the level of model control group was obviously higher than that of blank control group, the level of ascidians and Youjiangtang groups were obviously lower than that of model control group. Conclusions Ascidians has apparent glucose-lower effect, and has the obvious inhibition to plasma protein, liver, kidney, pancreas tissue protein,s nonenzymatic glycosylation induced by higher blood glucose. Key words:ascidians; blood glucose; proteins glycosylation; animal experiment 海鞘(Ascidian)属于脊索动物门(Chordates) ,尾索动物亚门(Urochordata) ,海鞘纲(class Ascidiacea)。它与尾索动物亚门的另外两个纲一起被称为被囊动物。全世界大约有2000种被囊动物,其中海鞘占大多数。近年来 ,对海鞘的研究取得重大进展 ,大量具有抗肿瘤、抗菌、抗病毒、抗炎、增加胰岛素