溪黄草配伍虎杖煎剂对大鼠实验性肝损伤保护作用.docVIP

溪黄草配伍虎杖煎剂对大鼠实验性肝损伤保护作用 　 作者：刘银花，陈秀琴，沈婕，艾文利 【摘要】 　　目的观察溪黄草配伍虎杖煎剂对大鼠肝损伤的协同保护作用及其机制。方法60只大鼠随机分为5组，即对照组（不用药）、模型组（CCl4损伤）、溪黄草组（CCl4损伤+溪黄草煎剂）、虎杖组（CCl4损伤+虎杖煎剂）、配伍组（CCl4损伤+溪黄草煎剂和虎杖煎剂）；用药8周后，用比色分析法测定大鼠血清天冬氨酸转氨酶（ALT）、丙氨酸转氨酶（AST）的活性；测定大鼠血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性及丙二醛（MDA）的含量。结果溪黄草组和虎杖组血清ALT，AST的活性、肝组织MDA明显降低；而肝组织SOD，GSH-Px的活性明显增高（Plt;0.01）；配伍组降低血清ALT，AST的活性，提高SOD，GSH-Px的活性，降低MDA含量的作用远较溪黄草组和虎杖组明显（Plt;0.01）。结论溪黄草水煎剂配伍虎杖水煎剂对CCl4所致大鼠急性肝损伤有协同保护作用，优于单味药物的保肝作用。 【关键词】 溪黄草 虎杖 大鼠 肝损伤 　　Abstract:ObjectiveTo research the protective effect and mechanism of Xihuangcao with Huzhang against chemical hepatic injury induced by perchlormethane in wistar rats. MethodsThe liver injury was induced by perchlormethane,then 60 wistar rats were divided into five groups-three of them treated by Xihuangcao, Huzhang and Xihuangcao with Huzhang espectively, and the other groups as control and control without treatment. After 8 weeks, the ALT，AST, SOD，GSH-Px activities and MDA level in serum were measured. ResultsIn all groups treated with Xihuangcao or Huzhang especially ,the ALT,AST activities and MDA level in serum were obviously decreased, the SOD，GSH-Px activities were obviously increased(Plt;0.01); the effects in Xihuangcao with Huzhang were better than those in Xihuangcao or Huzhang.ConclusionXihuangcao with Huzhang has a good protective effect against acute liver injury induced by perchlormethane ,better than Xihuangcao or Huzhang, the mechanism may be related to inhibiting the lipid peroxidation. 　　Key words:Xihuangcao; Huzhang; Rats; Liver injury 　　溪黄草为唇形科香茶菜属植物钱纹香茶菜Rsbdosia lophanthoides Hara.的干燥全根。具有抗肿瘤、抗菌、消炎及对肝脏的保护作用，现多用于治疗黄疸性肝炎、急性胆囊炎等疾病［1］。虎杖为蓼科蓼属多年生草本植物虎杖Polygonum cuspidatum Sieb.et Zucc.的干燥根和茎，具有祛风利湿、祛痰止咳、清热解毒、活血化淤、保肝利胆等作用［2］。本实验建立CCl4致大鼠急性肝损伤模型，观察溪黄草水煎剂配伍虎杖水煎剂对肝损伤的保护作用，并探讨其协同作用机理。 　　1 材料与方法 　　1.1 动物 　　选用健康Wistar大鼠，雌雄不拘，体重180～220 g，由广东省韶关粤北人民医院实验动物中心提供。 　　1.2 药材和试剂 　　溪黄草、虎杖采集于广东省韶关翁源县，经韶关药检所鉴定后，洗净风干，粉碎成粗粉，水煎，提取滤过液制备成4 g/ml的水煎剂备用。SOD，MDA，GSH-Px试剂盒，购自南京建成生物技术研究所；四氯化碳，分析纯，上海申鹤化学品有限公司产