琼玉膏抑制肝癌细胞HBxAg表达及对原发性肝癌防治作用.doc

琼玉膏抑制肝癌细胞HBxAg表达及对原发性肝癌防治作用 　 ; 作者：陈孝银; 魏春山, 童光东 毛慧君, 夏芳, 江振友 毕业论文 【关键词】; 肝癌;,琼玉膏;,HBxAg 毕业论文 　　Preventive and therapeutic effects of qiongyugao on hepatocellular carcinoma via inhibitionoftheexpressionofHBxAg in hepatic carcinoma cells 毕业论文 　　[Abstract]; AIM: To investigate the effects of qiongyugao on the expression of hepatitis B x antigen (HBxAg) in BALB/cnu mice into which human hepatic carcinoma cells were transplanted, and to analyze its specific mechanism in prophylaxis and treatment of hepatocellular carcinoma. METHODS: A nude mouse model with the transplantation of human hepatic carcinoma cells was established to observe the preventive and therapeutic effects of qiongyugao on the body weight and tumor weight of the mice. The expression of HBxAg in tumor and liver tissue wsa detected by immunohistochemical studies. RESULTS: Compared with model control group, prophylaxis and treatment with qiongyugao increased the body weight, depressed the tumor weight and inhibited HBxAg expression. The same efficacy was showed in both qiongyugao prophylaxis group and cyclophosphamide treatment group. CONCLUSION: Qiongyugao canslowdownthegrowthoftumorandinhibitthe expression of HBxAg, which may be an essential mechanism in prophylaxis and treatment of hepatocellular carcinoma. 毕业论文 　　[Keywords]hepatocellular carcinoma; qiongyugao; HBxAg 毕业论文 　　[摘 要]; 目的: 观察琼玉膏对人肝癌细胞移植裸鼠HBxAg表达的影响, 分析其通过影响HBxAg表达防治原发性肝癌的机制。方法: 建立人肝癌细胞移植裸鼠模型,; 观察琼玉膏预防及治疗对小鼠体质量、 癌组织质量的影响, 同时采用免疫组化法测定癌组织及肝脏中HBxAg的表达。结果: 与模型组相比, 琼玉膏预防及治疗后, 裸鼠的体质量增长迅速, 癌组织质量偏低, 癌组织及肝脏HBxAg低表达。琼玉膏预防与环磷酰胺治疗的效果相近。结论: 琼玉膏能减缓肿瘤的生长, 并抑制HBxAg的表达, 后者可能是其防治原发性肝癌的主要机制之一。 毕业论文 　　[关键词]肝癌; 琼玉膏; HBxAg ; 　　原发性肝癌是我国常见的恶性肿瘤之一, 其5年生存率仅为5%, 死亡率为2037/100000, 占恶性肿瘤死亡率的第2位[1]。目前, 原发性肝癌的病因和发病机制虽未完全肯定, 但已有调查显示, 乙型肝炎病毒（hepatitis B virus, HBV）是原发性肝癌发生的重要相关因素[2], 乙肝病毒的X基因是乙肝致癌过程中最主要的触发因子[3]。X基因位于HBV基因组不完全的双链DNA中负链上4个开放读码框（open reading frame, ORF）之一X区, 编码X蛋白, 即HBxAg。HBxAg是一反式激活因子, 在肝癌组织中高度表达, 可与TATA结合蛋白(TATA binding protein, TBP)作用, 激活病毒和细