甲状腺素对重症急性胰腺炎大鼠肠黏膜屏障功能保护作用.doc

甲状腺素对重症急性胰腺炎大鼠肠黏膜屏障功能保护作用 　【摘要】 ［目的］探讨重症急性胰腺炎时甲状腺激素的代谢异常与肠黏膜屏障损伤之间的关系，观察外源性甲状腺激素对肠黏膜屏障的保护作用.［方法］取Wistar大鼠分为假手术对照组、胰腺炎组及甲状腺素组，均应用牛磺胆酸钠制作重症急性胰腺炎模型，腹腔注射给予假手术对照组及胰腺炎组大鼠生理盐水2mL／kg,给予甲状腺素组大鼠甲状腺素15μg/kg.于给药12h时检测外周血FT3,FT4,促甲状腺激素和门静脉血内毒素及血清淀粉酶水平，并观察肠黏膜形态学变化.［结果］胰腺炎组大鼠血清FT3,FT4值显著降低，血浆内毒素及血清淀粉酶水平显著升高，肠黏膜损伤评分升高,与假手术对照组比较均有显著性差异；甲状腺素组大鼠内毒素水平与胰腺炎组比较显著降低，血清FT3,FT4值显著升高.［结论］胰腺炎时补充外源性甲状腺素对肠黏膜屏障功能具有保护作用. 【关键词】 胰腺炎 急性坏死性 肠黏膜 甲状腺激素 大鼠 ABSTRACT:OBJECTIVETo investigate the relationship between abnormal metabolism of thyroxin and injury of intestinal barrier, and observe the protective effects of exogenous thyroxin for intestinal barrier function in rats.METHODSWistar rats were randomly divided into 3 groups: sham operation group (SO), severe acute pancreatitis (SAP) group, thyroxin (TH) group. Serum FT3, FT4, TSH levels and serum amylase, plasma endotoxin level in the portal vein and injure of instestinal mucosal grade were examined on 12h.RESULTSCompare with SO group, in SAP group the serum FT3, FT4 levels were decreased, serum amyluse, plasma endotoxin level in portal vein were increased, and injure grade of intestinal mucosal were increased, and have significantly difference. Compare with SO group and TH group showed no significantly difference.CONCLUSIONSupplement of exogenous thyroxin can protect intestinal mucosal barrier in rats with severe acute pancreatitis. Key words:pancreatitis, acute necrotizing;intestinal mucosa;thyroid hormones;rats 目前因肠黏膜屏障功能损害所致的内源性感染仍是引发重症急性胰腺炎患者出现多脏器功能衰竭,甚至死亡的重要原因［1］.本研究通过建立重症急性胰腺炎动物模型，探讨重症急性胰腺炎时甲状腺素的代谢异常与肠黏膜屏障损伤之间的关系，观察了外源性甲状腺素对肠黏膜屏障的保护作用. 1 材料与方法 1.1 材料 实验动物取健康Wistar大鼠，雌雄不限，体重为200～250g，由延边大学医学部实验动物科提供.甲状腺素、鲎试剂盒及牛磺胆酸钠为SIGMA公司产品；甲状腺素放射免疫试剂盒及促甲状腺素放射免疫试剂盒均为天津原子能公司产品.主要仪器有D37520型高速冷冻离心机、UV2100型分光光度计、DFM96型多管放射免疫计数器及日本岛津产SP4430型全自动生物化学分析仪. 1.2 方法 1.2.1 分组 取42只Wistar大鼠，分为3个组，即假手术对照组10只，胰腺炎组15只，甲状腺素组17只. 1.2.2 模型制作 术前禁食12h，自由饮水.利用100mL/L的水合氯醛（3mL/kg）行腹腔注射麻醉,无菌条件下给胰腺炎组及甲状腺素组大鼠行腹正中线入腹术，充分游离脾脏，提起脾和胰腺尾部，于胰腺被膜下均匀注射30g/L牛磺胆酸钠1mL;假手术对照组大鼠仅做开腹手术，并行翻动