肾康丸对糖尿病肾病大鼠肾脏TGFβ1-Smad 7通路影响.docVIP

肾康丸对糖尿病肾病大鼠肾脏TGFβ1/Smad 7通路影响 　 【摘要】 目的 探讨肾康丸对糖尿病肾病（DN）大鼠肾脏TGFβ1/Smad 7信号通路的 影响 。 方法 用链脲佐菌素（STZ）腹腔注射法诱导建立大鼠糖尿病（DM）模型，模型成功后随机分为3组：模型对照组、开搏通组、肾康丸组，另设正常对照组。 治疗 8周后，取大鼠肾脏，采用免疫组化法观察各组大鼠肾脏组织纤连蛋白（FN）、转化生长因子β1（TGFβ1）和Smad 7蛋白表达的情况。结果 8周后，DN模型组大鼠与正常大鼠比较，肾脏组织中FN和TGFβ1蛋白表达明显增加，Smad 7蛋白表达明显减少。肾康丸和开搏通都可以减少FN和TGFβ1蛋白的表达，增加Smad 7蛋白的表达。结论 肾康丸可以抑制DN大鼠肾脏中TGFβ1/Smad信号传导通路的激活，减少ECM的沉积，从而延缓DN进程。 毕业论文 【关键词】 肾康丸；糖尿病肾病；TGFβ1/Smad信号通路 　　Abstract:Objective To explore the effects of the Shenkangwan pills on the TGFβ1/Smad 7 signaling pathway in the kidneys of the DN rats. Methods The diabetic rat models were induced by intraperitoneal injection of streptozotocin（STZ）and randomly divided into 3 groups：model control group；Capoten group and Shenkangwan group. Some other rats were used as normal control group. All rats were administered with corresponding drugs for 8 weeks. After the last administration，the kidneys of the rats were taken out. The protEin expression of FN, TGFβ1 and Smad 7 were detected with the method of immune histochemistry. Results The renal tissue protEIn expressions of FN and TGFβ1 in the rats of DN model group were increased than those in the rats of normal group. While the protein expression of Smad 7 was decreased distinctly in these rats’ kidneys. Compared with those in the rats of the DN model group，the protein expressions of FN and TGFβ1 in the rats of Shenkangwan and capoten group were decreased markedly. While the protein expression of Smad 7 was increased distinctly in these rats kidneys. Conclusion Shenkangwan could restrain the activation of TGFβ1/Smad signaling pathway in the renal tissue of DN rats and reduced the synthesis and secretion of the ECM， which would stay the course of the DN. 　　Key words:Shenkangwan pills；diabetic nephropathy；TGFβ1/Smad signaling pathway 毕业论文 　　糖尿病肾病（diabetic nephropathy，DN）是糖尿病（diabetes mellitus，DM）患者临床常见而难治的微血管并发症，是导致终末期肾病（ESRD）和DM患者死亡的主要原因［1］。如何有效预防DN发生 发展 ,具有重要意义。肾康丸在临床治疗DN患者多年，疗效确切。临床观察和动物实验 研究 都表