胎儿肠道Cajal间质细胞发育.docVIP

胎儿肠道Cajal间质细胞发育 　　　作者：吴小红，王宝西，王茂贵 【关键词】 Cajal间质细胞 Development of interstitial cells of Cajal in fetal intestines 　　【Abstract】 AIM: To study the development and distribution of interstitial cells of Cajal (ICC) in fetal intestines. METHODS: Twelve samples of gestation ICC from 3-4 months were collected, ckit immunohistochemistry were used in the experiment. RESULTS: ①ICC was distributed in circular muscle layer, longitudinal muscle layer and the myenteric plexus. ②The distribution of ICC varied throughout the intestines with different gestational ages and intestines layers. The number of ICC increased with the gestational age. ③ICC appeared first along the myenteric plexus, then in muscle layer and then in deep muscular plexus. ④With the increase of gestational age, the cell body enlarged and its foot processes elongated. ICC formed network gradually. CONCLUSION: The development of ICC varies throughout the intestines with the different gestational ages and intestines layers. ICC are located primarily in the myenteric plexus of Auerbach. The number of ICC increases and ICC form network gradually with the increase of gestational age. 　　【Keywords】 interstitial cells of Cajal; intestines; distribution; fetal 　　【摘要】 目的: 研究胎儿肠道Cajal间质细胞（interstitial cells of Cajal，ICC）分布及发育情况. 方法: 搜集中止妊娠3~8 mo胎儿标本12份，采用酪氨酸激酶受体（ckit）抗体免疫组织化学方法标记胎儿肠道ICC. 结果: ①ICC分布于肠肌间神经丛、纵肌和环肌层面；②不同胎龄胎儿标本，不同层面ICC分布存在差异，随胎龄增加，ICC数目增多；③ICC的呈现按一定时间顺序，肠肌间神经丛，ICC呈现最早，肌内次之，深肌丛较晚；④ICC胞体逐渐增大，足突延长并互相连接，形成细胞间网络结构. 结论： 胎儿肠道ICC主要分布肠肌间神经丛周围，不同胎龄ICC分布及发育存在差异，不同层面ICC分布不同，随着胎龄增加，ICC数目增多，逐渐形成网络结构. 　　【关键词】 Cajal间质细胞；肠；分布；胎儿 　　0引言 　　Cajal间质细胞（interstitial cells of Cajal，ICC)来源于间充质远祖细胞，该细胞表达kit和平滑肌球蛋白重链，在妊娠后期ICC和纵形平滑肌谱系开始分叉［1］. ICC为分布于消化道自主神经末梢与平滑肌细胞之间的特殊类型细胞，其细胞膜表面存在许多神经递质受体如ckit，CD34等. ICC功能既由其数目、分布、免疫活性决定，又受神经，肌细胞功能影响. ICC与肌细胞间的缝隙连接对其功能发挥起重要作用. ICC分布异常、数目减少、功能降低可能是引起慢传输型便秘，慢性假性肠梗阻等胃肠动力性疾病的主要因素. 目前缺乏对ICC发育研究的资料，我们拟探讨胎儿ICC发育、分布，对明确胃肠动力性疾病病因，可能具有重要意义. 　　1材料和方法 　　1.1材料 　　12份中止妊娠之胎儿标本,根据胎龄分为3组: 3～4 mo 3份、5～6 mo 3份、7～8 mo 6份. 主要