胰腺癌组织中血管内皮生长因子及肿瘤相关巨噬细胞及淋巴结转移.doc

胰腺癌组织中血管内皮生长因子及肿瘤相关巨噬细胞及淋巴结转移 　【摘要】 目的 研究胰腺癌组织中血管内皮生长因子(vascular endothelial growth factor,VEGF)表达和肿瘤相关巨噬细胞(tumourassociated macrophages，TAMs)计数与淋巴结转移的关系。方法 采用免疫组织化学染色，在光镜下对VEGF阳性细胞评分及TAMs计数进行分析。结果 胰腺癌组织中VEGF表达阳性率明显高于正常组织(Plt;0.01)；淋巴结转移组VEGF的表达和TAMs计数较未转移组明显增高(Plt;0.05)；VEGF的表达与胰腺癌临床病理分期无明显关系(Pgt;0.05)；TAMs计数与胰腺癌临床病理分期有明显关系(Plt;0.01)；VEGF的表达与TAMs计数呈正相关(r=0.663，Plt;0.01)。结论 VEGF和TAMs的表达均与胰腺癌淋巴结转移有密切关系。 【关键词】 胰腺肿瘤； 血管内皮生长因子； 肿瘤相关巨噬细胞； 淋巴结转移 【Abstract】 Objective To investigate relationship of expressions of vascular endothelial growth factor (VEGF) and counting of tumourassociated macrophages (TAMs) and discuss their correlation with lymphatic metastasis in pancreatic cancer. Methods After immunohistochemical staining, light microscope was employed to determine expressions of VEGF and TAMs. Statistical analysis was performed by using SPSS software. Results The positive rate of VEGF in pancreatic cancer was 51% (18/35), which was significantly higher than that of normal tissues (Plt;0.01).(1) The scores of VEGF and the number of TAMs in metastatic cancer were significantly higher than those in the cancer without metastasis (Plt;0.05). (2) There was no statistically significant correlation between scores of VEGF and tumor staging (Pgt;0.05). (3) There was statistically significant relationship between number of TAMs and tumor staging (Plt;0.01). (4) The expressions of VEGF were positively correlated with number of TAMs (r=0.663, Plt;0.01).Conclusions Expressions of VEGF and number of TAMs may relate to the lymphatic metastasis of pancreatic cancer. 【Key words】 Pancreatic cancer; Vascular endothelial growth factor; Tumourassociated macrophages; Lymphatic metastasis 胰腺癌是临床上较为少见的恶性肿瘤，其晚期常有淋巴结转移。在肿瘤细胞的恶性增殖和转移的过程中，血管内皮生长因子(vascular endothelial growth factor，VEGF)被认为是最重要的因子之一。有研究发现在肿瘤中浸润的巨噬细胞——肿瘤相关巨噬细胞(tumourassociated macrophages， TAMs)也与淋巴结转移有密切关系［1］。本研究采用免疫组织化学方法对两者与淋巴结转移的关系进行分析，现报道如下。 1 材料与方法 1.1 临床资料 收集1999-2004年中国医科大学附属一院及沈阳医学院附属奉天医院手术切除胰腺癌标本35例，其中男24例，女11例；