芒果甙对痤疮主要致病菌抑菌作用探究.docVIP

芒果甙对痤疮主要致病菌抑菌作用探究 　 作者：叶海洪，农朝赞，黄婵娟，黄之虎，潘莉莉 农少云，韦仕喻，郭凌霄，吴玉清 【摘要】 目的探讨芒果甙对痤疮主要致病菌的抑菌作用，为深度开发芒果树药用植物资源提供研究依据。方法采用液体试管法，进行金黄色葡萄球菌的常规培养及痤疮丙酸杆菌的厌氧培养，按试管稀释法调整细菌浓度;以红霉素为对照，观察芒果甙对痤疮丙酸杆菌及金黄色葡萄球菌的最小抑菌浓度(MIC)。结果芒果甙对痤疮主要致病菌有一定的抑菌作用但不如红霉素明显，芒果甙对痤疮丙酸杆菌的MIC为8mmol/L;对金黄色葡萄球菌的MIC为20mmol/L;而红霉素对痤疮丙酸杆菌的MIC为160μmol/L;对金黄色葡萄球菌的MIC为20μmol/L;100μmol/L的芒果甙能把红霉素的MIC浓度从160μmol/L下降至0.160μmol/L。结论芒果甙可降低红霉素的最低抑菌浓度，两者配伍使用，可降低红霉素的使用剂量。 【关键词】 芒果甙;丙酸杆菌，痤疮;葡萄球菌，金黄色;最低抑菌浓度;微生物敏感性试验 　Abstract:ObjectiveTo investigate suppressive effects of Mangiferin on Propionibacterium Acnes in vitro, so as to provide research evidence for further exploiting the drug plant resources of Mango trees.MethodsA fluid test tubemethod was used to perform normal Staphylococcus aureus culture and Staphylococcus aureus anaerobic culture, and the cell concentrations were regulated in tube　dilution series. The erythromycin was used as control, the minimal inhibitory concentration (MIC) of Mangiferin on Propionibacterium Acnes and Staphylocous aureus were observed.ResultsMangiferin can inhibit the Propionibacterium acnes to some extent, but not as sensitive as erythromycin. The MIC of Mangiferin toPropionibacterium acnes is 8 mmol/L and to Staphylocous aureus is 20mmol/L. TheMIC of erythromycin to Propionibacterium acnes is 160 μmol/L and to Staphylocous aureus is 20 μmol/L. The Mangiferin can change the MIC of erythromycin; 100μmol/L of Mangiferin can reduce the MIC of the erythromycin from 160μmol/L to 0.160 μmol/L.ConclusionMangiferin can reduce the MIC of　erythromycin. When combined with erythromycin, it can reduce the dose usage of erythromycin 　　Key words:Mangiferin; Propionibacterium acnes; Staphylococcus aureus; minimal inhibitory concentration (MIC); microbial sensitivity tests 　　痤疮俗称“粉刺”、“青春痘”，是一种由多因素引起的常见慢性毛囊、皮脂腺炎症性皮肤病。有研究[1]表明在痤疮皮损中存在有多种细菌，其中痤疮丙酸杆菌及金黄色葡萄球菌为主要致病菌，在痤疮的不同发展阶段都存在。痤疮丙酸杆菌存在于痤疮发生的多个环节中，它能诱发或加重痤疮症状，用药物减少皮肤毛囊中主要致病菌的数量是目前治疗痤疮的主要途径之一。芒果甙是一种四羟基吡啶的碳糖甙，属双苯吡酮类化合物。有文献报道[2,3]芒果甙具有抗过氧化脂质、抗炎抗菌的作用。笔者用我实验室