花梨木挥发油β-环糊精包合物制备及稳定性探究.docVIP

花梨木挥发油β-环糊精包合物制备及稳定性探究 　 作者：韦吉崇，纪明慧，舒火明，郭飞燕，李婷，唐康 【摘要】 目的制备花梨木挥发油β-环糊精包合物并考察包合物的稳定性。方法在不同溶媒的作用下以饱和水溶液搅拌法包合花梨木挥发油β-环糊精包合物，以花梨木挥发油含量为测定指标，分别对花梨木挥发油β-环糊精包合物和混合物进行强光照射、高温、挥发性实验并通过DSC-TGA对包合物进行了热稳定性研究。结果在醋酸乙酯为溶媒的条件下，β-环糊精包合挥发油的包合率最高，包合率可达75.8%。在光、热等因素影响下，包合物中花梨木挥发油含量变化不大，而混合物中挥发油含量均明显下降。包合物热稳定性比混合物好，热分解反应为一级反应。结论确定了醋酸乙酯为合适的溶媒，花梨木挥发油β-环糊精包合物具有一定的抗光照性、热稳定性，其稳定性优于单纯花梨木挥发油。 【关键词】 花梨木； 挥发油； β-环糊精； 包合物； 稳定性 ChinaAbstract：ObjectiveTo study the preparation and stability of inclusion compound of volatile oil extracted from Dalbergia odorifera T. chen.MethodsThe inclusion compound of β-cyclodextrin was prepared in different menstruums by saturated aqueous solution mixing method. With the volatile oil content of Dalbergia odorifera as an index, the stability of the inclusion compound was examined under strong light, high temperature and volatilizing, respectively. In addition, the heat stability of the inclusion complex was investigated by DSC-TGA.ResultsThe highest inclusion ratio was obtained when ethyl acetate was used as a menstruum, and the yield could reach 75.8%.In addition, the volatile oil content of the inclusion complex was not much sensitive to light and heat. In the mixture, however, it decreased considerably. The inclusion complex was more thermostable than the mixture and the thermodecomposion was a one-order reaction.ConclusionEthyl acetate is an appropriate menstruum for the inclusion, and the volatile oil in inclusion complex has higher stability than that of original volatile oil as a result of good light and heat stabilities of the inclusion complex. 　　Key words：Dalbergia odorifera； Volatile oil； β-cyclodextrin； Inclusion compound； Stability 　　花梨木挥发油是豆科植物花梨木Dalbergia odorifera T. chen的树干和根的干燥心材经水蒸气蒸馏法提取得到的淡黄色油状物，其具有降血压、舒筋活脉、抗血栓形成等药理作用［1～3］ ，但该挥发油水溶性差，味苦不易口服，且是挥发性的液体，不便于保存。为了减少制剂过程中挥发油的损失，稳定其药用成分，探索防止花梨木挥发油氧化分解、挥发，提高花梨木挥发油稳定性的方法，对其制剂的研制是十分必要的［4～6］ 。本实验将利用β-环糊精对花梨木挥发油进行包合，并考察了包合物和混合物的稳定性，为花梨木挥发油制成新型药剂提供理论依据。 　　1 仪器与材料 　　1.1 仪器 　　KDM型调温电热套（山东省堙城永兴仪器厂）；TU-1901型双