花椒体内外抗肿瘤作用及其机制初步探究.docVIP

花椒体内外抗肿瘤作用及其机制初步探究 　 作者：袁太宁, 王艳林, 汪鋆植 【摘要】 目的研究花椒挥发油体内外抗小鼠H22肝癌细胞作用及其免疫学机制。方法花椒挥发油按不同时间（24，48，72 h）及不同浓度（0. 5～8 mg/ml）分别处理H22细胞，MTS法分析细胞的增殖速度，用流式细胞仪测定细胞凋亡及细胞周期。建立H22荷瘤小鼠模型，观察小鼠肿瘤的生长情况,计算肿瘤生长抑制率；ELISA法检测花椒挥发油处理后小鼠血清中IL-2和IL-12的活性。结果4 mg/ml花椒挥发油处理H22细胞72 h可显著地抑制H22细胞增殖，1 mg/ml花椒挥发油处理H22细胞72 h即可导致亚凋亡峰的出现，且G0/G1期细胞增多, S期和G2/M期细胞减少，花椒挥发油对小鼠实体瘤生长具有显著抑制作用，但不能提高荷瘤小鼠血清中IL-2和IL-12的水平。结论花椒挥发油可抑制H22细胞增殖并激发细胞凋亡，但不能通过提高机体的免疫功能发挥抗肿瘤作用。 【关键词】 花椒； H22细胞； 凋亡； 免疫功能 　　Abstract：ObjectiveTo study the anti-tumer effect and its immunological mechanism of the essential oil of zanthoxylum in vivo and in vitro. Methods Treating H22 cells at different time and with different concentration of the essential oil of zanthoxylum, MTS was used to assay the cell proliferation, flow cytometry was used to assay the cell apoptosis and cell cycles. The H22 BALB/C xenograft tumor modes were established. The anti-tumor effect of zanthoxylum was observed and tumor growth inhibitory rate (IR) was calculated. The content of serum IL-2 and IL-12 were examined by ELISA assay. ResultsTreating H22 cells 72h by 4mg/ml essential oil of zanthoxylum resulted in remarkable inhibition of the cell proliferation.Treating H22 cells 72h by 1mg/ml essential oil of zanthoxylum resulted in appearance of sub-G1 and increase G0/G1 phase cells and decrease S phase and G2/M phase cells. The essential oil of zanthoxylum inhibited the growth of tumor in tumor-bearing mice. However, essential oil of zanthoxylum could not increase the contents of murine serum IL-2 and IL-12. ConclusionThe essential oil of zanthoxylum inhibits proliferation and induces cell apoptosis of H22 cells, but it can not exert the anti-tumor efficiency through reinforcing the immune function. 　　Key words： Zanthoxylum ; H22 cells; Apoptosis; Immune function 　　花椒属一种温热类中草药，辛,热,有小毒，归脾、肾两经,功能温中止痛,解毒杀虫，常用于治疗脾胃虚寒症。花椒还是深受民众喜爱的重要食用性香料，其资源在我国分布很广,安全性高。国内外研究报道花椒对消炎、镇痛、杀菌、抗病毒、抗寄生虫、抗真菌、抗氧化等均具有较强的作用［1～5］。随着对花椒提纯和药理作用的深入研究,发现花椒还具有抗肿瘤作用,但目前对花椒抗肿瘤的研究还不够深入,其体内的抗肿瘤作用研究则更少。为了