苦参碱及顺铂合用对小鼠肝癌移植瘤影响.docVIP

苦参碱及顺铂合用对小鼠肝癌移植瘤影响 　 作者：黄宏思，黄赞松，黄卫彤，黄衍强 【摘要】 　　目的观察苦参碱联合顺铂对小鼠H22肝癌移植瘤生长的抑制作用。方法复制H22肝癌小鼠移植瘤模型。将40只接种H22肝癌细胞昆明小鼠随机分成4组：对照组、苦参碱组（MW）、顺铂组（DDP）、联合组（MW +DDP）。治疗期间，观察小鼠的毒副反应以及生存质量。实验19d后，处死全部小鼠，剥离皮下肿瘤，称小鼠肿瘤重量，计算出抑瘤率。结果MW单用组的H22肝癌平均瘤重为（0.89±0.27）g，明显低于对照组的（1.23±0.36）g（Plt;0.01）。MW与DDP合用组的H22肝癌平均瘤重为（0.17±0.11）g，均明显低于MW单用组（Plt;0.01）和DDP单用组（Plt;0.01）；其抑瘤率达86.2%，明显高于MW单用组（Plt;0.01）和DDP单用组（Plt;0.01）。联合治疗组小鼠毒副反应明显低于DDP组，生存质量优于DDP组。结论苦参碱和顺铂对小鼠H22肝癌移植瘤的生长具有协同抑制作用，并能降低顺铂毒副反应，提高生存质量。 【关键词】 苦参碱 顺铂注射液 H22肝癌移植瘤 　　Abstract：ObjectiveTo study the inhibitory action of matrine (MW) combined with cisplatin(DDP) on H22 liver cancer in mice.MethodsH22 liver cancer cells were inoculated into the mammary fatty pad of mice to establish H22 liver cancer model，40 mice bearing highly metastatic H22 liver cancer cells were randomly divided into four groups: control group，DDP group，MW group and MW+ DDP group. Different treatments were served from day 7 after transplantation and all mice were sacrificed after 19 days. During the treatments，the toxic side reaction of chemotherapy and the life quality were observed. Subcutaneous tumors were processed for tumor weight，and tumor growth inhibition rate and tumor necrosis rate of each group were calculated. ResultsThe tumor weight of MW group was(0.89±0.27)g，compared with the control group (1.23±0.36)g, there was a significant difference（Plt;0.01）．The tumor weight of MW+ DDP group was （0.17±0.11）g，compared with MW group and DDP group,it was significantly lower（Plt;0.01）and （Plt;0.01）．The tumor inhibition rate of MW+ DDP group was 86.2%，which was significantly hrgher MW group and DDP group, there were significant differences（Plt;0.01）. Compared with DDP group, the toxic side reaction of chemotherapy in combination group was significantly lower，but life quality was higher. Conclusioncombined application MW and DDP has stronger inhibition on tumor growth than single use，and they have a synergic anticancer effect to H22 liver cancer in mice，decrease toxic side