a new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an otx2 enhancer between mouse and chick extraembryonic endoderm新方法使转染小鸡胚胎的内胚层揭示otx2增强器之间的监管保护鼠标和小鸡胚胎外的内胚层.pdfVIP

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a new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an otx2 enhancer between mouse and chick extraembryonic endoderm新方法使转染小鸡胚胎的内胚层揭示otx2增强器之间的监管保护鼠标和小鸡胚胎外的内胚层.pdf

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a new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an otx2 enhancer between mouse and chick extraembryonic endoderm新方法使转染小鸡胚胎的内胚层揭示otx2增强器之间的监管保护鼠标和小鸡胚胎外的内胚层

BMC Developmental Biology BioMed Central Methodology article Open Access A new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an Otx2 enhancer between mouse and chick extraembryonic endoderm Amanda Albazerchi 1,2, Olivier Cinquin1,3 and Claudio D Stern* 1 Address: 1Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT. UK, 2Laboratory of Genetics, 425-G Henry Mall, University of Wisconsin, Madison, WI 53706, USA and 3Deparment of Biochemistry, University of Wisconsin, Madison, WI 53706, USA Email: Amanda Albazerchi - albazerchi@; Olivier Cinquin - cinquin@; Claudio D Stern* - c.stern@ucl.ac.uk * Corresponding author Published: 2 April 2007 Received: 1 December 2006 Accepted: 2 April 2007 BMC Developmental Biology 2007, 7:25 doi:10.1186/1471-213X-7-25 This article is available from: /1471-213X/7/25 © 2007 Albazerchi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The mouse anterior visceral endoderm (AVE) and the chick hypoblast are thought to have homologous roles in the early stages of neural induction and primitive streak formation. In mouse, many regulatory elements directing gene expression to the AVE have been identified. However, there is no technique