a new spreadsheet method for the analysis of bivariate flow cytometric data一个新的电子表格二元流仪数据分析的方法.pdfVIP

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a new spreadsheet method for the analysis of bivariate flow cytometric data一个新的电子表格二元流仪数据分析的方法.pdf

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a new spreadsheet method for the analysis of bivariate flow cytometric data一个新的电子表格二元流仪数据分析的方法

BMC Cell Biology BioMed Central Methodology article Open Access A new spreadsheet method for the analysis of bivariate flow cytometric data George Tzircotis, Rick F Thorne and Clare M Isacke* Address: Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK Email: George Tzircotis - george.tzircotis@icr.ac.uk; Rick F Thorne - rick.thorne@icr.ac.uk; Clare M Isacke* - clare.isacke@icr.ac.uk * Corresponding author Published: 22 March 2004 Received: 19 December 2003 Accepted: 22 March 2004 BMC Cell Biology 2004, 5:10 This article is available from: /1471-2121/5/10 © 2004 Tzircotis et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: A useful application of flow cytometry is the investigation of cell receptor-ligand interactions. However such analyses are often compromised due to problems interpreting changes in ligand binding where the receptor expression is not constant. Commonly, problems are encountered due to cell treatments resulting in altered receptor expression levels, or when cell lines expressing a transfected receptor with variable expression are being compared. To overcome this limitation we have developed a Microsoft Excel spreadsheet that aims to automatically and effectively