a novel asymmetric 3d in-vitro assay for the study of tumor cell invasion一种新型非对称的3 d研究体外测定肿瘤细胞入侵.pdfVIP

BMC Cancer BioMed Central Technical advance Open Access A novel asymmetric 3D in-vitro assay for the study of tumor cell invasion Vera Brekhman and Gera Neufeld* Address: Cancer Research and vascular Biology Center, The Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, PO Box 9679, 1 Efron St, Haifa, 31096, Israel Email: Vera Brekhman - verab@tx.technion.ac.il; Gera Neufeld* - gera@tx.technion.ac.il * Corresponding author Published: 30 November 2009 Received: 11 June 2009 Accepted: 30 November 2009 BMC Cancer 2009, 9:415 doi:10.1186/1471-2407-9-415 This article is available from: /1471-2407/9/415 © 2009 Brekhman and Neufeld; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The induction of tumor cell invasion is an important step in tumor progression. Due to the cost and slowness of in-vivo invasion assays, there is need for quantitative in-vitro invasion assays that mimic as closely as possible the tumor environment and in which conditions can be rigorously controlled. Methods: We have established a novel asymmetric 3D in-vitro invasion assay by embedding a monolayer of tumor cells between two layers of collagen. The cells were then allowed to invade the upper and lower