a study on the two binding sites of hexokinase on brain mitochondria两个结合位点的研究己糖激酶对大脑线粒体.pdfVIP
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a study on the two binding sites of hexokinase on brain mitochondria两个结合位点的研究己糖激酶对大脑线粒体
BMC Biochemistry BioMed Central
Research article Open Access
A study on the two binding sites of hexokinase on brain
mitochondria
Abolfazl Golestani†1, Hassan Ramshini†2 and Mohsen Nemat-Gorgani*3
Address: 1Department of Clinical Biochemistry, School of Medicine, Medical Sciences/University of Tehran, Tehran 14155-6447, Iran, 2Institute
of Biochemistry and Biophysics, University of Tehran, Tehran 13145-1384, Iran and 3Stanford Genome Technology Center, Stanford University,
Palo Alto, CA, USA
Email: Abolfazl Golestani - golsetan@tums.ac.ir; Hassan Ramshini - hramshini@ibb.ut.ac.ir; Mohsen Nemat-
Gorgani* - mohsenn@
* Corresponding author †Equal contributors
Published: 20 October 2007 Received: 22 April 2007
Accepted: 20 October 2007
BMC Biochemistry 2007, 8:20 doi:10.1186/1471-2091-8-20
This article is available from: /1471-2091/8/20
© 2007 Golestani et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Type I hexokinase (HK-I) constitutes the predominant form of the enzyme in the
brain, a major portion of which is associated with the outer mitochondrial membrane involving two
sets of binding sites. In addition to the glucose-6-phosphate (G6P)-sensitive site (Type A), the
enzyme is bound on a second set of sites (Type B) which are, while insensitive to G6P, totally
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