原创力文档advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation肿瘤进展建立和分析三维spheroid-based目标验证和药物评价的功能分析.pdfVIP
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advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation肿瘤进展建立和分析三维spheroid-based目标验证和药物评价的功能分析
Advances in establishment and analysis of three-
dimensional tumor spheroid-based functional
assays for target validation and drug evaluation
Vinci et al.
Vinci et al. BMC Biology 2012, 10:29
/1741-7007/10/29 (22 March 2012)
Vinci et al. BMC Biology 2012, 10:29
/1741-7007/10/29
METHODOLOGY ARTICLE Open Access
Advances in establishment and analysis of three-
dimensional tumor spheroid-based functional
assays for target validation and drug evaluation
1 1 1 1 1 1 1
Maria Vinci , Sharon Gowan , Frances Boxall , Lisa Patterson , Miriam Zimmermann , William Court , Cara Lomas ,
Marta Mendiola2, David Hardisson3 and Suzanne A Eccles1*
Abstract
Background: There is overwhelming evidence that in vitro three-dimensional tumor cell cultures more accurately
reflect the complex in vivo microenvironment than simple two-dimensional cell monolayers, not least with respect
to gene expression profiles, signaling pathway activity and drug sensitivity. However, most currently available three-
dimensional techniques are time consuming and/or lack reproducibility; thus standardized and rapid protocols are
urgently needed.
Results: To address this requirement, we have developed a versatile toolkit of reproducible three-dimensional
tumor spheroid models for dynamic, automated, quantitative imaging and analysis that are compatible with
routine high-throughput preclinical studies. Not only do these microplate methods measure three-dimensional
tumor growth, but they have also been significantly enhanced to facilitate a range of functional assays
exemplifying additional key hallmarks of ca
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