an experimental protocol for mimicking pathomechanisms of traumatic brain injury in mice一个实验性的协议模仿pathomechanisms创伤性脑损伤的老鼠.pdfVIP

Albert-Weißenberger et al. Experimental Translational Stroke Medicine 2012, 4:1 /content/4/1/1 REVIEW Open Access An experimental protocol for mimicking pathomechanisms of traumatic brain injury in mice 1* 2 3 1 3 Christiane Albert-Weißenberger , Csanád Várrallyay , Furat Raslan , Christoph Kleinschnitz and Anna-Leena Sirén Abstract Traumatic brain injury (TBI) is a result of an outside force causing immediate mechanical disruption of brain tissue and delayed pathogenic events. In order to examine injury processes associated with TBI, a number of rodent models to induce brain trauma have been described. However, none of these models covers the entire spectrum of events that might occur in TBI. Here we provide a thorough methodological description of a straightforward closed head weight drop mouse model to assess brain injuries close to the clinical conditions of human TBI. Keywords: closed head injury, traumatic brain injury, neurobehavioural deficits, astrocyte, microglia, neurons 1 Introduction axonal swelling, axoplasmic ovoid retraction balls and Traumatic brain injury (TBI) is a result of an outside expression of amyloid beta peptides [17]. The original force causing immediate mechanical disruption of brain weight drop model in rats by Feeney [18,19] was optimi- tissue and delayed pathogenic events that can exacerbate mized to produce an open-head brain injury whereas the injury (reviewed by [1]). It represents a leading cause the model developed by Shohami [20-22] produces a of death and disability in the industr