原创力文档anti-apoptotic gene transcription signature of salivary gland neoplasms唾腺肿瘤的抗凋亡基因转录的签名.pdfVIP
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anti-apoptotic gene transcription signature of salivary gland neoplasms唾腺肿瘤的抗凋亡基因转录的签名
Gomes et al. BMC Cancer 2012, 12:61
/1471-2407/12/61
RESEARCH ARTICLE Open Access
Anti-apoptotic gene transcription signature of
salivary gland neoplasms
1* 2 2 3
Carolina Cavaliéri Gomes , Vanessa Fátima Bernardes , Marina Gonçalves Diniz , Luiz De Marco and
Ricardo Santiago Gomez2
Abstract
Background: Development of accurate therapeutic approaches to salivary gland neoplasms depends on better
understanding of their molecular pathogenesis. Tumour growth is regulated by the balance between proliferation
and apoptosis. Few studies have investigated apoptosis in salivary tumours relying almost exclusively on
immunohistochemistry or TUNEL assay. Furthermore, there is no information regarding the mRNA expression
profile of apoptotic genes in salivary tumors. Our objective was to investigate the quantitative expression of BCL-2
(anti-apoptotic), BAX and Caspase3 (pro-apoptotic genes) mRNAs in salivary gland neoplasms and examine the
association of these data with tumour size, proliferative activity and p53 staining (parameters associated with a
poor prognosis of salivary tumours patients).
Methods: We investigated the apoptotic profile of salivary neoplasms in twenty fresh samples of benign and
seven samples of malignant salivary neoplasms, using quantitative real time PCR. We further assessed p53 and ki-67
immunopositivity and obtained clinical tumour size data.
Results: We demonstrated that BCL-2 mRNA is overexpressed in salivary neoplasms, leading to an overall anti-
apoptotic profile. We also found an association between the anti-apoptotic index (BCL-2/BAX) with p53
immunoexpression. A higher proliferative activity was found in the malignant tumours. In addition, tumour size
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