原创力文档antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of albizia adianthifolia (mimosoideae)乙酸乙酯提取物的抗氧化和抗菌活动,分数和化合物的干树皮albizia adianthifolia(mimosoideae).pdfVIP
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antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of albizia adianthifolia (mimosoideae)乙酸乙酯提取物的抗氧化和抗菌活动,分数和化合物的干树皮albizia adianthifolia(mimosoideae)
Tamokou et al. BMC Complementary and Alternative Medicine 2012, 12:99
/1472-6882/12/99
RESEARCH ARTICLE Open Access
Antioxidant and antimicrobial activities of ethyl
acetate extract, fractions and compounds from
stem bark of Albizia adianthifolia (Mimosoideae)
1* 2 1,3 2 2
Jean de Dieu Tamokou , Deke James Simo Mpetga , Paul Keilah Lunga , Mathieu Tene , Pierre Tane
and Jules Roger Kuiate1
Abstract
Background: Albizia adianthifolia is used traditionally in Cameroon to treat several ailments, including infectious
and associated diseases. This work was therefore designed to investigate the antioxidant and antimicrobial activities
of ethyl acetate extract, fractions and compounds isolated from the stem bark of this plant.
Methods: The plant extract was prepared by maceration in ethyl acetate. Its fractionation was done by column
chromatography and the structures of isolated compounds were elucidated using spectroscopic data in
conjunction with literature data. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity
(TEAC) assays were used to detect the antioxidant activity. Broth micro-dilution method was used for antimicrobial
test. Total phenol content was determined spectrophotometrically in the extracts by using Folin–Ciocalteu method.
Results: The fractionation of the extract afforded two known compounds: lupeol ( 1) and aurantiamide acetate
(2) together with two mixtures of fatty acids: oleic acid and n-hexadecanoic acid (B1); n-hexadecanoic acid,
octadecanoic acid and docosanoic acid (B2). Aurantiamide acetate was the most active compound. The total
phenol concentration expressed as gallic acid equivalents (GAE) was found to vary from 1.50 to
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