antiproliferative and cell apoptosis-inducing activities of compounds from buddleja davidii in mgc-803 cells化合物的抗增殖和细胞凋亡诱导活动从buddleja davidii mgc - 803细胞.pdfVIP

Wu et al. Cell Division 2012, 7:20 /content/7/1/20 RESEARCH Open Access Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells † † * Jian Wu , Wenshi Yi , Linhong Jin, Deyu Hu and Baoan Song Abstract Background: Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural products for their activity against the human gastric cancer cell line Mgc-803 and the human breast cancer cell line Bcap-37. Results: Ten compounds were extracted and isolated from B. davidii, among which colchicine was identified in B. davidii for the first time. The inhibitory activities of these compounds were investigated in Mgc-803, Bcap-37 cells in vitro by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, and the results showed that luteolin and colchicine had potent inhibitory activities against the growth of Mgc-803 cells. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in + - Mgc-803 cells. The results also showed that the percentages of early apoptotic cells (Annexin V /PI , where PI is propidium iodide) and late apoptotic cells (Annexin V+/PI+) increased in a dose- and time-dependent manner. After 36 h of incubation with luteolin at 20 μM, the percentages of cells were approximately 15.4% in early apoptosis and 43.7% in late apoptosis; after 36 h of incubation with colchicine at 20 μM, the corresponding values were 7.7% and 35.2%, respec