auto-protective redox buffering systems in stimulated macrophagesauto-protective刺激巨噬细胞氧化还原缓冲系统.pdfVIP

BMC Immunology BioMed Central BMC Immunology x 2002, 3 Research article Auto-protective redox buffering systems in stimulated macrophages Pierre-Jacques Ferret, Emmanuelle Soum, Olivier Negre and Didier Fradelizi* Address: Université René Descartes, Paris V Inserm U477, Hôpital Cochin, 75014 Paris, France E-mail: Perret Pierre-Jacques - pjferret@; Soum Emmanuelle - fradeli@cochin.inserm.fr; Negre Olivier - fradeli@cochin.inserm.fr; Didier Fradelizi* - fradeli@cochin.inserm.fr *Corresponding author Published: 12 March 2002 Received: 12 October 2001 Accepted: 12 March 2002 BMC Immunology 2002, 3:3 This article is available from: /1471-2172/3/3 © 2002 Pierre-Jacques et al; licensee BioMed Central Ltd. Verbatim copying and redistribution of this article are permitted in any medium for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Macrophages, upon encounter with micro-organisms or stimulated by cytokines, produce various effector molecules aimed at destroying the foreign agents and protecting the organism. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are front line molecules exerting strong cytotoxic activities against micro-organisms and many cells, including macrophages themselves. Using cells of the murine macrophage cell line (RAW 264.7) stimulated in vitro with lipopolysaccharide (LPS) and/or interferon (IFN-), which induce strong endogenous NO production, we examined by which mechanisms a