原创力文档high mobility group box 1 potentiates the pro-inflammatory effects of interleukin-1β in osteoarthritic synoviocytes高机动组框1强化interleukin-1β的促炎作用在骨关节炎synoviocytes.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
García-Arnandis et al. Arthritis Research Therapy 2010, 12:R165
/content/12/4/R165
RESEARCH ARTICLE Open Access
High mobility group box 1 potentiates the
pro-inflammatory effects of interleukin-1b in
osteoarthritic synoviocytes
1 1,2 3 4 4
Isabel García-Arnandis , Maria Isabel Guillén , Francisco Gomar , Jean-Pierre Pelletier , Johanne Martel-Pelletier ,
Maria José Alcaraz1*
Abstract
Introduction: High mobility group box 1 (HMGB1) is released by necrotic cells or secreted in response to
inflammatory stimuli. Extracellular HMGB1 may act as a pro-inflammatory cytokine in rheumatoid arthritis. We have
recently reported that HMGB1 is released by osteoarthritic synoviocytes after activation with interleukin-1beta (IL-
1b) The present study investigated the role of HMGB1 in synovial inflammation in osteoarthritis (OA).
Methods: HMGB1 was determined in human synovium using immunohistochemistry, comparing normal to OA.
OA synoviocytes were incubated with HMGB1 at 15 or 25 ng/ml in the absence or presence of IL-1b (10 ng/ml).
Gene expression was analyzed by quantitative PCR and protein expression by Western Blot and ELISA. Matrix
metalloproteinase (MMP) activity was studied by fluorometric procedures and nuclear factor (NF)-B activation by
transient transfection with a NF-B-luciferase plasmid.
Results: In the normal synovium, HMGB1 was found in the synovial lining cells, sublining cells, and in the vascular
wall cells. The distribution of HMGB1 in OA synovium was similar but the number of HMGB1 positive cells was
higher and HMGB1 was also present in infiltrated cells. In normal synovial membrane cells, HMGB1 was found
mostly in the nuclei, whereas in OA, HMGB1 was generally found mostly
您可能关注的文档
- crystal structure of n,n,n’,n’-tetra-[(3,5-dimethyl-1-pyrazolyl) methyl]-para-phenylenediamine晶体结构的n,n,n ',n '四-[(3 5-dimethyl-1-pyrazolyl)甲基]-para-phenylenediamine.pdf
- cytokine mrna and protein expression in primary-culture and repeated-passage synovial fibroblasts from patients with rheumatoid arthritis细胞因子mrna和蛋白表达在初代培养和repeated-passage从类风湿性关节炎患者滑膜成纤维细胞.pdf
- cytotoxic triterpenoid saponins from the roots of platycodon grandiflorum细胞毒性桔梗根的三萜皂甙.pdf
- cytomegalovirus reactivation and associated outcome of critically ill patients with severe sepsis巨细胞病毒激活和相关危重严重脓毒症患者的结果.pdf
- axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatinaxon-bearing期间生成的连续和axon-less水平细胞亚型鸡视网膜发展u2014u2014卵泡敏感,过分生长的祖细胞.pdf
- cytoprotective efficacy of amifostine against radiation- induced rectal toxicity objective and subjective grading scales for radiomucositiscytoprotective amifostine对抗辐射的功效,引起直肠毒性radiomucositis客观和主观评分尺度.pdf
- decreases in procalcitonin and c-reactive protein are strong predictors of survival in ventilator-associated pneumonia减少原降钙素和c反应蛋白与机械通气相关肺炎是生存的强烈预报.pdf
- derivatives of 10,16-dihydroxyhexadecanoic acid isolated from tomato (solanum lycopersicum) as potential material for aliphatic polyesters衍生品的16-dihydroxyhexadecanoic酸分离番茄(茄属植物lycopersicum)作为潜在的脂肪族聚酯材料.pdf
- design strategies of fluorescent biosensors based on biological macromolecular receptors设计策略的基于生物大分子受体荧光生物传感器.pdf
- detection and classification of human body odor using an electronic nose人体气味检测和分类使用电子鼻子.pdf
- high mobility group box-1 protein in patients with suspected community-acquired infections and sepsis a prospective study高机动组盒1蛋白在疑似社区获得性感染和脓毒症患者的前瞻性研究.pdf
- high quality bergamot oil from greece chemical analysis using chiral gas chromatography and larvicidal activity against the west nile virus vector高质量的佛手柑油从希腊使用手性化学分析气相色谱法对西尼罗病毒向量和杀灭幼虫的活动.pdf
- high prevalence of syphilis among demobilized child soldiers in eastern congo a cross-sectional study高的梅毒患病率复员儿童兵在刚果东部一个横断面研究.pdf
- high prevalence of lipoatrophy in pre-pubertal south african children on antiretroviral therapy a cross-sectional study脂肪萎缩pre-pubertal南非孩子的横断面研究抗逆转录病毒治疗.pdf
- high plasma uric acid concentration causes and consequences高血浆尿酸浓度的原因和后果.pdf
- high resolution crystal structures of the cerebratulus lacteus mini-hb in the unligated and carbomonoxy states高分辨率的晶体结构cerebratulus lacteus mini-hb unligated和carbomonoxy状态.pdf
- high rates of albuminuria but not of low egfr in urban indigenous australians the druid study高蛋白尿但不低的表皮生长因子受体在城市土著澳大利亚人德鲁伊的研究.pdf
- high resolution discovery and confirmation of copy number variants in 90 yoruba nigerians高分辨率的拷贝数变异的发现和确认90年尼日利亚约鲁巴语.pdf
- high risk behaviors of injection drug users registered with harm reduction programme in karachi, pakistan高危险行为的注射吸毒者在减少危害注册项目在卡拉奇,巴基斯坦.pdf
- high resolution marine magnetic survey of shallow water littoral area高分辨率海洋磁力测量浅海沿岸地区.pdf
文档评论（0）