highly sensitive and miniaturized fluorescence detection system with an autonomous capillary fluid manipulation chip高度敏感和小型荧光检测系统自主毛细管液处理芯片.pdfVIP

Micromachines 2012, 3, 462-479; doi:10.3390/mi3020462 OPEN ACCESS micromachines ISSN 2072-666X /journal/micromachines Article Highly Sensitive and Miniaturized Fluorescence Detection System with an Autonomous Capillary Fluid Manipulation Chip Mingjin Yao 1,*, Girish Shah 2 and Ji Fang 1 1 Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71270, USA; E-Mail: jfang@ 2 College of Pharmacy, University of Louisiana at Monroe, Monroe, LA 71209, USA; E-Mail: shah@ * Author to whom correspondence should be addressed; E-Mail: mya003@. Received: 10 April 2012; in revised form: 27 April 2012 / Accepted: 9 May 2012 / Published: 10 May 2012 Abstract: This paper presents a novel, highly sensitive and ultra-small fluorescent detection system, including an autonomous capillary fluid manipulation chip. The optical detector integrates a LED light source, all necessary optical components, and a photodiode with preamplifier into one package of about 2 cm × 2 cm × 2 cm. Also, the low-cost and simple pumpless microfluidic device works well in sample preparation and manipulation. This chip consists of capillary stop valves and trigger valves which are fabricated by lithography and then bonded with a polydimethylsiloxane-ethylene oxide polymer polydimethylsiloxane (PEO-PDMS) cover. The contact angle of the PEO-PDMS can be adjusted by changing the concentration of the PEO. Hence, the fluidic chip can achieve func