how does long-term odor deprivation affect the olfactory capacity of adult mice长期剥夺气味如何影响成年老鼠的嗅觉能力.pdfVIP

Angely and Coppola Behavioral and Brain Functions 2010, 6:26 /content/6/1/26 R E S E A R C H Open Access Research How does long-term odor deprivation affect the olfactory capacity of adult mice? Cathy J Angely and David M Coppola* Abstract Background: Unilateral naris occlusion (UNO) has been the most common method of effecting stimulus deprivation in studies of olfactory plasticity. However, despite the large corpus on the effects of this manipulation, dating back to the 19th century, little is known about its behavioral sequela. Here we report the results of standard olfactory habituation and discrimination studies on adult mice that had undergone perinatal UNO followed by adult contralateral olfactory bulbectomy (bulb-x). Methods: The olfactory performance of UNO mice was compared to matched controls that had unilateral bulb-x but open nares. Both habituation and discrimination (operant) experiments employed a protocol in which after successful dishabituation or discrimination to dilute individual odors (A = 0.01% isoamyl acetate; B = 0.01% ethyl butyrate; each v/ v in mineral oil), mice were challenged with a single odor versus a mixture comparison (A vs. A + B). In a series of tests the volume portion of Odor B in the mixture was systematically decreased until dishabituation or discrimination thresholds were reached. Results: For the habituation experiment, UNOs (n = 10) and controls (n = 9) dishabituated to a 10% mixture of Odor B in Odor A after being habituated to A alone, while both groups failed to show differential responding to a 2% mixture of B in A. However, the UNO groups increased investigation durations for the 2% mixture approached significance (p 0.06). A replication of this study (7 controls 8 UNOs) confirmed that controls did not differentiate Odor A and a 2% mixture of B in A but