high throughput method to quantify anterior-posterior polarity of t-cells and epithelial cells高通量方法量化前后极性下的t细胞和上皮细胞.pdfVIP

Viruses 2011, 3, 2396-2411; doi:10.3390/v3122396 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article High Throughput Method to Quantify Anterior-Posterior Polarity of T-Cells and Epithelial Cells 1 2 1 1 Charletha V. Irvin-Wilson , Justin Y. Newberg , Kathleen Kong , Ronald T. Javier and Susan J. Marriott 1,* 1 Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA; E-Mails: irvinwil@ (C.V.I.-W.); kong@ (K.K.); rjavier@ (R.T.J.) 2 Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA; E-Mail: newberg@ * Author to whom correspondence should be addressed; E-Mail: susanm@; Tel.: +1-713-798-4440; Fax: +1-713-798-4435. Received: 23 September 2011; in revised form: 16 November 2011 / Accepted: 23 November 2011 / Published: 28 November 2011 Abstract: The virologic synapse (VS), which is formed between a virus-infected and uninfected cell, plays a central role in the transmission of certain viruses, such as HIV and HTLV-1. During VS formation, HTLV-1-infected T-cells polarize cellular and viral proteins toward the uninfected T-cell. This polarization resembles anterior-posterior cell polarity induced by immunological synapse (IS) formation, which is more extensively characterized th