multifunctional polypeptide eqcn sensors probing the cysteamine-glutathione film permeability with hg(ii) ions多功能多肽eqcn传感器探测cysteamine-glutathione电影渗透率与hg(ii)离子.pdfVIP

Sensors 2008, 8, 7224-7240; DOI: 10.3390/s8117224 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Multifunctional Polypeptide EQCN Sensors: Probing the Cysteamine-Glutathione Film Permeability with Hg(II) Ions Maria Hepel 1,2,* and Julia Dallas 1 1 Department of Chemistry, State University of New York at Potsdam, Potsdam, NY 13676, USA 2 Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14260, USA * Author to whom correspondence should be addressed; E-mail: hepelmr@; Tel.: +1-315- 267-2267; Fax: +1-315-267-3170 Received: 16 October 2008; in revised form: 10 November 2008 / Accepted: 13 November 2008 / Published: 14 November 2008 Abstract: Multifunctional films are the basis of biosensors and play an important role in the emerging field of nanobioelectronics. In this work, films of a tripeptide glutathione (GSH) immobilized on a self-assembled monolayer of cysteamine (CA-SAM) on a quartz crystal Au piezosensor have been synthesized and characterized using electrochemical quartz crystal nanogravimetry (EQCN) with a Hg(II) ion probe. It has been found that in contrast to previously studied Au/GSH films, the Au/CA-GSH films strongly hinder the formation of Hg0 with bulk properties while still allowing for relatively easy permeation by Hg(II) ions. This results in complete disappearance of the sharp Hg0 electrodissolution peak which is observed on bare Au and Au/GSH piezosensors. The multiple-peak