myosins xi-k, xi-1, and xi-2 are required for development of pavement cells, trichomes, and stigmatic papillae in arabidopsis肌凝蛋白xi-kξ1,ξ2是路面的发展所需细胞,毛状体,丑恶的拟南芥的乳头状突起.pdfVIP

Ojangu et al. BMC Plant Biology 2012, 12:81 /1471-2229/12/81 RESEARCH ARTICLE Open Access Myosins XI-K, XI-1, and XI-2 are required for development of pavement cells, trichomes, and stigmatic papillae in Arabidopsis 1* 1 1 1 2 1 1 Eve-Ly Ojangu , Krista Tanner , Pille Pata , Kristel Järve , Carola L Holweg , Erkki Truve and Heiti Paves Abstract Background: The positioning and dynamics of vesicles and organelles, and thus the growth of plant cells, is mediated by the acto-myosin system. In Arabidopsis there are 13 class XI myosins which mediate vesicle and organelle transport in different cell types. So far the involvement of five class XI myosins in cell expansion during the shoot and root development has been shown, three of which, XI-1, XI-2, and XI-K, are essential for organelle transport. Results: Simultaneous depletion of Arabidopsis class XI myosins XI-K, XI-1, and XI-2 in double and triple mutant plants affected the growth of several types of epidermal cells. The size and shape of trichomes, leaf pavement cells and the elongation of the stigmatic papillae of double and triple mutant plants were affected to different extent. Reduced cell size led to significant size reduction of shoot organs in the case of triple mutant, affecting bolt formation, flowering time and fertility. Phenotype analysis revealed that the reduced fertility of triple mutant plants was caused by delayed or insufficient development of pistils. Conclusions: We conclude that the class XI myosins XI-K, XI-1 and XI-2 have partially redundant roles in the growth of shoot epidermis. Myosin XI-K plays more important role whereas myosins XI-1 and XI-2 have minor roles in the determination of size and shape of epider