neonatal phosphate nutrition alters in vivo and in vitro satellite cell activity in pigs磷酸新生儿营养改变体内和体外猪卫星细胞活动.pdfVIP

Nutrients 2012, 4, 436-448; doi:10.3390/nu4060436 OPEN ACCESS nutrients ISSN 2072-6643 /journal/nutrients Article Neonatal Phosphate Nutrition Alters in Vivo and in Vitro Satellite Cell Activity in Pigs Lindsey S. Alexander 1, Brynn S. Seabolt 1, Robert P. Rhoads 2 and Chad H. Stahl 1,* 1 Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA; E-Mails: lindsey.alexander1@ (L.S.A.); bsseabol@ (B.S.S.) 2 Department of Animal Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; E-Mail: rhoadsr@ * Author to whom correspondence should be addressed; E-Mail: chad_stahl@; Tel.: +1-919-513-1512; Fax: +1-919-515-6884. Received: 29 April 2012; in revised form: 22 May 2012 / Accepted: 24 May 2012 / Published: 31 May 2012 Abstract: Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4) deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW) were pair-fed liquid diets that were either PO4 adequate (0.9% total P), supra-adequate (1.2% total P) in PO requirement or deficient (0.7% total P) in PO content 4 4 for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs