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new trends in biosensors for organophosphorus pesticides对有机磷农药生物传感器的新趋势
Sensors 2001, 1, 60-74
sensors
ISSN 1424-8220
© 2001 by MDPI
/sensors
New Trends in Biosensors for Organophosphorus Pesticides
Nicole Jaffrezic-Renault
IFoS-UMR CNRS 5621 Ecole Centrale de Lyon, BP 163 69131 ECULLY Cedex, France.
Tel: (33) 4 72 18 62 43. Fax: (33) 4 78 33 15 77. E-mail: Nicole.Jaffrezic@ec-lyon.fr
Received: 13 July 2001 / Accepted: 18 July 2001 / Published: 24 July 2001
Abstract: Biosensors are sensitive and can be used as disposable sensors for environmental
control. These biosensors are based either on inhibition of acyl cholinesterases
acetylcholinesterase or butyrylcholinesterase) by organophosphorus compounds or on
inhibition of enzymes phosphatases (acid or alkaline) or on direct detection of
organophosphorus compounds by organophosphorus hydrolase. The state-of-the-art of the
three types of biosensors will be presented in this paper and a particular development will be
done on our work on ENFET (Enzymatic Field Effect Transistor) biosensors.
Keywords: Inhibition, Acetylcholinesterase, Butyrylcholinesterase, Phosphatases,
Organophosphorus hydrolase, Multivariate correspondence analysis
Introduction
Organophophorus compounds are, among the known substances, the most toxic. They are used as
pesticides, insecticides and chemical war agents. The high toxicity of organophosphorus neurotoxics
and their large use in modern agriculture practices, has increased public concerns. They have incited
the developm
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