nitric oxide detection with glassy carbon electrodes coated with charge-different polymer films一氧化氮与玻璃碳电极上涂上一层检测收取不同的聚合物薄膜.pdfVIP

Sensors 2005, 5, 161-170 sensors ISSN 1424-8220 © 2005 by MDPI t/sensors Nitric Oxide Detection with Glassy Carbon Electrodes Coated with Charge-different Polymer Films Osamu Ikeda *, Kumiko Yoshinaga and Jianping Lei Department of Chemistry, Faculty of Science, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1192, Japan * Author to whom correspondence should be addressed. E-mail: osamu@kenroku.kanazawa-u.ac.jp Received: 9 June 2004 / Accepted: 28 October 2004 / Published: 26 April 2005 Abstract: Trace amounts of nitric oxide (NO) have been determined in aqueous phosphate buffer solutions (pH=7.4) by using a glassy carbon electrode coated with three charge-different polymer films. The glassy carbon electrode was coated first with negatively charged Nafion film containing tetrakis(pentafluorophenylporphyrin) iron(III) chloride (Fe(III)TPFPP) as the NO oxidation catalyst, and then with positively charged poly(acrylamide-co-diallyldimethylammonium chloride) (PADDA) and with neutral poly(dimethylsiloxane) (silicone) at the outermost layer. This polymer-coated electrode showed an excellent selectivity towards NO against possible concomitants in blood such as nitrite, ascorbic acid, uric acid, and dopamine. All current ratios between each concomitant and NO at the cyclic voltammogram was in 10-3 ~ 10-4. This type of electrode showed a detection limit of 80 nM for NO. It was speculated from the electrochemical study in methanol that high-valent o