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Sensors 2009, 9, 3713-3744; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems Clarissa Lui 1,*, Nathaniel C. Cady 2 and Carl A. Batt 3 1 Department of Biomedical Engineering / Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA 2 College of Nanoscale Science and Engineering / University at Albany State University of New York, 255 Fuller Rd., Albany, NY 12203, USA; E-Mail: ncady@ (N.C.C.) 3 Department of Food Science / Cornell University, 312 Stocking Hall, Ithaca, NY 14853, USA; E-Mail: cab10@ (C.A.B.) * Author to whom correspondence should be addressed; E-Mail: csl42@; Tel. +1-607-255-7902; Fax: +1-607-255-8741 Received: 9 April 2009; in revised form: 12 May 2009 / Accepted: 18 May 2009 / Published: 18 May 2009 Abstract: The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid- based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase