photonic biosensor assays to detect and distinguish subspecies of francisella tularensis光子生物传感器化验检测和区分亚种的土拉杆菌内.pdfVIP

Sensors 2011, 11, 3004-3019; doi:10.3390/s110303004 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Communication Photonic Biosensor Assays to Detect and Distinguish Subspecies of Francisella tularensis Kristie L. Cooper 1, Aloka B. Bandara 2, Yunmiao Wang 1, Anbo Wang 1 and Thomas J. Inzana 2,* 1 Center for Photonics Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; E-Mails: klcooper@ (K.L.C.); kathy618@ (Y.W.); awang@ (A.W.) 2 Center for Molecular Medicine Infectious Diseases, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; E-Mail: abandara@ (A.B.B.) * Author to whom correspondence should be addressed; E-Mail: tinzana@; Tel.: 540-231-5188; Fax: 540-231-5553. Received: 15 January 2011; in revised form: 15 February 2011 / Accepted: 2 March 2011 / Published: 7 March 2011 Abstract: The application of photonic biosensor assays to diagnose the category-A select agent Francisella tularensis was investigated. Both interferometric and long period fiber grating sensing structures were successfully demonstrated; both these sensors are capable of detecting the optical changes induced by either immunological binding or DNA hybridization. Detection was made possible by the attachment of DNA probes or immunoglobulins (IgG) directly to the fiber surface via layer-by-layer electrostatic self-assembly. An optical fiber biosensor was tested using a standard transmission mode long