phylogenetic relationships in genus arachis based on its and 5.8s rdna sequences落花生属的系统发育关系根据其和5.8 s rdna序列.pdf

Bechara et al. BMC Plant Biology 2010, 10:255 /1471-2229/10/255 RESEARCH ARTICLE Open Access Phylogenetic relationships in genus Arachis based on ITS and 5.8S rDNA sequences 1 2 3 4 5 Marcelo D Bechara , Márcio C Moretzsohn , Darío A Palmieri , Jomar P Monteiro , Maurício Bacci Jr , 6 2 4 2* Joaquim Martins Jr , José FM Valls , Catalina R Lopes , Marcos A Gimenes Abstract Background: The genus Arachis comprises 80 species and it is subdivided into nine taxonomic sections (Arachis, Caulorrhizae, Erectoides, Extranervosae, Heteranthae, Procumbentes, Rhizomatosae, Trierectoides, and Triseminatae). This genus is naturally confined to South America and most of its species are native to Brazil. In order to provide a better understanding of the evolution of the genus, we reconstructed the phylogeny of 45 species using the variation observed on nucleotide sequences in internal transcribed spacer regions (ITS1 and ITS2) and 5.8 S of nuclear ribosomal DNA. Results: Intraspecific variation was detected, but in general it was not enough to place accessions of the same species in different clades. Our data support the view that Arachis is a monophyletic group and suggested Heteranthae as the most primitive section of genus Arachis . The results confirmed the circumscriptions of some sections (Caulorrhizae, Extranervosae), but raised questions about others. Sections Erectoides, Trierectoides and Procumbentes were not well defined, while sections Arachis and Rhizomatosae seem to include species that could be moved to different sections. The division of section Arachis into A and B genome species was also observed in the phylog