phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth植物化学的分析和methanolic提取物的抗菌活动叶、茎和根从不同种类的labisa pumila benth.pdfVIP

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phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth植物化学的分析和methanolic提取物的抗菌活动叶、茎和根从不同种类的labisa pumila benth.pdf

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phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth植物化学的分析和methanolic提取物的抗菌活动叶、茎和根从不同种类的labisa pumila benth

Molecules 2011, 16, 4438-4450; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Phytochemical Analysis and Antimicrobial Activities of Methanolic Extracts of Leaf, Stem and Root from Different Varieties of Labisa pumila Benth Ehsan Karimi 1, Hawa Z.E. Jaafar 1,* and Sahida Ahmad 2 1 Departments of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia 2 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia * Author to whom correspondence should be addressed; E-Mails: hawazej@; hawazej@.my. Received: 14 March 2011; in revised form: 2 May 2011 / Accepted: 4 May 2011 / Published: 27 May 2011 Abstract: A local herb, Kacip Fatimah, is famous amongst Malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. Therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of Labisia pumila Benth. Total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. Leaves of var. pumila exhibited significantly higher total saponin content than var. alata and lanceolata, with values of 56.4, 43.6 and 42.3 mg diosgenin equivalent/g dry weight, respective