positive- and negative-acting regulatory elements contribute to the tissue-specific expression of inner no outer, a yabby-type transcription factor gene in arabidopsis积极,negative-acting监管元素有助于组织的内在没有外在的表达,yabby-type转录因子基因在拟南芥.pdfVIP

Simon et al. BMC Plant Biology 2012, 12:214 /1471-2229/12/214 RESEARCH ARTICLE Open Access Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of INNER NO OUTER, a YABBY-type transcription factor gene in Arabidopsis 1 1,2 1 1,3 1* Marissa K Simon , Luis A Williams , Kristina Brady-Passerini , Ryan H Brown and Charles S Gasser Abstract Background: The INNER NO OUTER (INO) gene, which encodes a YABBY-type transcription factor, specifies and promotes the growth of the outer integument of the ovule in Arabidopsis. INO expression is limited to the abaxial cell layer of the developing outer integument of the ovule and is regulated by multiple regions of the INO promoter, including POS9, a positive element that when present in quadruplicate can produce low-level expression in the normal INO pattern. Results: Significant redundancy in activity between different regions of the INO promoter is demonstrated. For specific regulatory elements, multimerization or the addition of the cauliflower mosaic virus 35S general enhancer was able to activate expression of reporter gene constructs that were otherwise incapable of expression on their own. A new promoter element, POS6, is defined and is shown to include sufficient positive regulatory information to reproduce the endogenous pattern of expression in ovules, but other promoter regions are necessary to fully suppress expression outside of ovules. The full-length INO promoter, but not any of the INO promoter deletions tested, is able to act as an enhancer-blocking insulator to prevent the ectopic activation of expression by the 35S enhancer. Sequence conservation between the promoter regions of Arabidopsis tha