原创力文档pterins as sensors of response to the application of fe3+-dextran in piglets蝶呤作为响应的传感器在小猪fe3 +右旋糖酐的应用.pdfVIP
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pterinsassensorsofresponsetotheapplicationoffe3-dextraninpiglets蝶呤作为响应的传感器在小猪fe3右旋糖酐的应用
Sensors 2010, 10, 890-900; doi:10.3390/s100100890
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Pterins as Sensors of Response to the Application of Fe3+-Dextran
in Piglets
Miriam Smutna 1,*, Martin Svoboda 2 and Klara Breinekova 3
1 Department of Biochemistry, Chemistry and Biophysics, Faculty of Veterinary Hygiene and
Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, Brno, 612 42,
Czech Republic
2 Clinic of Pig Diseases, Faculty of Veterinary Medicine, University of Veterinary and
Pharmaceutical Sciences Brno, Palackeho 1/3, Brno, 612 42, Czech Republic;
E-Mail: svobodama@vfu.cz
3 Synthon s. r. o., Brnenska 32, Blansko 678 17, Czech Republic; E-Mail:
Klara.Breinekova@synthon.cz
* Author to whom correspondence should be addressed; E-Mail: smutnam@vfu.cz;
Tel.: +420-541-562-602; Fax: +420-541-562-790.
Received: 16 November 2009; in revised form: 30 December 2009 / Accepted: 14 January 2010 /
Published: 22 January 2010
Abstract: The aim of the presented study was to assess the effect of a single administration
of Fe3+-dextran on immune cell counts and pterin biomolecule production as novel sensors
of the piglets immune system activation, and to determine concentrations of cortisol, a
traditional hormonal biosensor of the stress response. Pterins (neopterin and biopterin) in
the piglets blood serum were analyzed by separation using reversed-phase HPLC. A single
dose of Fe3+-dextran produced a special stress situatio
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