quantification of the tissue-culture induced variation in barley (hordeum vulgare l.)量化组织培养诱导变异的大麦(大麦l .).pdfVIP

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quantification of the tissue-culture induced variation in barley (hordeum vulgare l.)量化组织培养诱导变异的大麦(大麦l .).pdf

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quantification of the tissue-culture induced variation in barley (hordeum vulgare l.)量化组织培养诱导变异的大麦(大麦l .)

BMC Plant Biology BioMed Central Research article Open Access Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.) Piotr T Bednarek*†1, Renata Orłowska†2, Robert MD Koebner†3 and Janusz Zimny†1 Address: 1Plant Breeding Acclimatization Institute, 05-870 Błonie, Radzików, Poland, 2UWM, Olsztyn, Poland and 3John Innes Centre, Colney Lane, Norwich NR4 7UH, UK Email: Piotr T Bednarek* - p.bednarek@.pl; Renata Orłowska - renata.orlowska@.pl; Robert MD Koebner - mockbeggars@; Janusz Zimny - j.zimny@.pl * Corresponding author †Equal contributors Published: 2 March 2007 Received: 1 August 2006 Accepted: 2 March 2007 BMC Plant Biology 2007, 7:10 doi:10.1186/1471-2229-7-10 This article is available from: /1471-2229/7/10 © 2007 Bednarek et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: When plant tissue is passaged through in vitro culture, many regenerated plants appear to be no longer clonal copies of their donor genotype. Among the factors that affect this so-called tissue culture induced variation are explant genotype, explant tissue origin, medium composition, and the length of time in culture. Variation is understood to be generated via a combination of ge