quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet量化使用无色结晶紫过氧化氢在含铁的解决方案.pdfVIP

GEOCHEMICAL TRANSACTIONS VOLUME 6, NUMBER 3 SEPTEMBER 2005 Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet Corey A. Cohna Department of Geosciences and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York 11794-2100 Aimee Pak Department of Chemistry and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York 11794-2100 Daniel Strongin Department of Chemistry, Beury Hall 201, 1901 North 13th Street, Temple University, Philadelphia, Pennsylvania 19122 and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York 11794-2100 Martin A. Schoonen Department of Geosciences and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York 11794-2100 Received 24 February 2005; accepted 26 April 2005; published 14 June 2005 Hydrogen peroxide is present in many natural waters and wastewaters. In the presence of FeII, this species decomposes to form hydroxyl radicals, that are extremely reactive. Hence, in the presence of FeII, hydrogen peroxide is difficult to detect because of its short lifetime. Here, we show an expanded use of a hydrogen peroxide quantification technique using leuco crystal violet LCV for solutions of varying p H and iron concentration. In the presence of the biocatalyst peroxidase, LCV is oxidized by hydrogen peroxide, forming a colored crystal violet ion CV+, which is stable for days. The LCV method uses standard equipment and allows for detection at the low microM concentration level. Results show strong p H dependence with max